| 引用本文:朱巍明1，刘伟2，刘源1.基于网络药理学研究复健片治疗缺血性脑卒中的作用机制[J].世界中医药,2021,(19):. |
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| 基于网络药理学研究复健片治疗缺血性脑卒中的作用机制 |
| Action and Mechanism of Fujian Tablet in the Treatment of Ischemic Stroke Based on Network Pharmacology |
| 投稿时间：2020-08-20 |
| DOI：10.3969/j.issn.1673-7202.2021.19.010 |
| 中文关键词: 中医药 复健片 缺血性脑卒中 网络药理学 蛋白质 靶点 机制 通路 |
| English Keywords:Traditional Chinese medicine Fujian tablet Ischemic Stroke Network Pharmacology Protein Target Mechanism Pathway |
| 基金项目:国家自然科学基金项目（81273701）；泰山学者青年专家计划项目（tsqn201909186）；山东省自然科学基金项目（ZR2019MH056） |
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| 中文摘要: |
| 目的：借助网络药理学平台技术探究复健片治疗缺血性脑卒中（IS）的作用机制。方法：利用中药系统药理学与分析平台(TCMSP)、中医药综合数据库(TCMID)、Batman-TCM数据库筛选出复健片中何首乌、桑寄生、决明子、淫羊藿、海马的活性成分及其靶点；利用Uniprot数据库对复健片中靶点信息进行校对；运用GeneCards数据库获取IS的疾病靶点基因；通过Cytoscape 3.7.2软件及STRING 11.0数据库构建蛋白质-蛋白质相互作用(PPI)网络并进行生物富集分析。结果：获取复健片治疗IS的共45个活性成分和144个靶点，关键靶点涉及IL6、IL1β、TNF、VEGFA、NOS3等；IS相关靶点1 485个；获得300条GO生物过程和84条相关信号通路，涉及PI3K-AKT信号通路、TNF信号通路、cGMP-PKG信号通路、cAMP信号通路、核因子κB信号通路等。结论：复健片是通过多成分、多靶点、多通路相互作用的机制来达到治疗IS的目的，通过本研究为复健片面向临床进一步推广应用提供理论支持，并为未来研究提供思路。 |
| English Summary: |
| To investigate the mechanism of Fujian Tablets in the treatment of ischemic stroke(IS) with the help of Internet pharmacology platform technology.Methods:TCMSP，TCMID and Batmann-TCM databases were used to screen the active components and their targets of Polygonum multiflorum，sangjis-heng，Cassia obtusifolia，epimedium and hippocampus in Fujian Tablets;the target information in Fujian T-ablets was proofread by UniProt database; the disease target genes of IS were obtained by GeneCard-s database; Cytoscape 3.7.2 software and STRING 11.0 database were used to construct PPI network and conduct bio-enrichment analysis.Results:A total of 45 active components and 144 targets were obtained，including IL6，IL1 β，TNF，VEGFA，NOS3，etc.; 1485 IS related targets; 300 GO biological processes and 84 related signal pathways were obtained，involving PI3K-AKT signaling pathway，TNF signaling pathway，cGMP-PKG signaling pathway，cAMP signaling pathway，NF-κB signaling pathway，etc.Conclusion:Fujian Tablet through the mechanism of multi-component，multi-target，multi-channel interaction can achieve the purpose of treating IS.This research provides theoretical support for the further clinical application of Fujian tablets，and provides ideas for future experimental research. |
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