| 引用本文:王思艺1，孙丽蕴2.蒿秦化斑方对紫外线损伤角质形成细胞黏附分子及PI3K/AKTAKT/GSK-3β通路的影响[J].世界中医药,2021,(19):. |
|
| 蒿秦化斑方对紫外线损伤角质形成细胞黏附分子及PI3K/AKTAKT/GSK-3β通路的影响 |
| Effects of Haoqin-Huaban Formula on the Cell Adhesion Molecules and the PI3K/AKT/GSK-3β Pathway of an Acute Ultraviolet B Light HaCat-Cell Injury Model |
| 投稿时间：2020-11-27 |
| DOI：10.3969/j.issn.1673-7202.2021.19.012 |
| 中文关键词: 光敏感性皮肤病 蒿秦化斑方 蛋白激酶B 糖原合成酶激酶-3β 细胞黏附分子 人永生化角质形成细胞 紫外线B 紫外线损伤 |
| English Keywords:Photosensitive skin disease Haoqin-Huaban Formula AKT GSK-3β Cell adhesion molecule HaCat cells Ultraviolet B Ultraviolet injury |
| 基金项目:国家自然科学基金面上项目（81774309）；北京中医医院顺义医院院级课题（SYYJ-201806） |
|
| 摘要点击次数: 175 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的：观察蒿秦化斑方对HaCat细胞紫外线B（UVB）急性损伤模型细胞黏附分子及PI3K/AKT/GSK-3β通路的影响，探讨该方治疗光敏感性皮肤病的作用机制。方法：采用300 mJ/cm2 UVB建立HaCat细胞急性光损伤模型，使用不同剂量蒿秦化斑方干预，分为空白对照组、UVB模型组、及蒿秦化斑方高、中、低剂量组。采用RT-PCR法检测细胞黏附分子ICAM-1、ELAM-1、VCAM-1相对表达量，采用蛋白质免疫印迹法分析PI3K/AKT/GSK-3β通路相关节点p-AKT(Ser-473)、p-AKT(Thr-308)、GSK-3β、PI3K相对表达量。结果：UVB急性光损伤可有效减少HaCat细胞ICAM-1的表达（P<0.01），对VCAM-1及ELAM-1表达影响不显著；蒿秦化斑方高剂量组能有效抑制HaCat细胞3种细胞黏附分子的表达（P<0.05）；蒿秦化斑方中剂量组能有效抑制HaCat细胞内皮细胞白细胞黏附分子-1（ELAM-1）及血管细胞黏附分子-1（VCAM-1）的表达（P<0.01）。UVB急性光损伤对HaCat细胞AKT磷酸化、PI3K表达影响不显著，蒿秦化斑方低剂量处理对HaCat细胞AKT磷酸化具有促进作用（P<0.05）。UVB急性光损伤使HaCat细胞糖原合成酶激酶-3β（GSK-3β）表达水平升高（P<0.01），蒿秦化斑方高、中、低剂量处理均对HaCat细胞GSK-3β表达水平有所抑制（P<0.01）。结论：蒿秦化斑方能降低由UVB急性损伤造成的HaCat细胞ICAM-1、ELAM-1、VCAM-1等黏附分子的表达进而缓解局部炎症和迟发型超敏反应的发生，并且能够激活HaCat细胞PI3K/AKT/GSK-3β通路，在UVB造成的急性光损伤中起到抗氧化、抗凋亡和降低炎症反应的作用。 |
| English Summary: |
| To observe the effects of Haoqin-Huaban on the relative adhesion factors and the PI3K/AKT/GSK-3β pathway of an acute UVB light HaCat-cell injury model in order to further explore the mechanism of the treatment for photosensitive skin disorders.Methods:UVB light(300 mJ/cm2) was used to establish an acute light HaCat-cell injury model.The intervention was performed by using Haoqin-Huaban in various concentrations.The groups were divided into：a blank control group(C); an UVB model group(M); a high Haoqin-Huaban dosage group(G); medium Haoqin-Huaban dosage group(Z); low Haoqin-Huaban dosage group(D).Real-time PCR was conducted to detect the relative expressions of 3 cell adhesion molecules(CAMs)，i.e.，ICAM-1，ELAM-1 and VCAM-1.Western Blotting was implemented to analyze the relative expressions of p-AKT(Ser-473)，p-AKT(Thr-308)，GSK-3β，and PI3K，which were all related nodes of the PI3K/AKT/GSK-3β pathway.Western Blot method was used to analyze the relative expression of p-AKT(Ser-473)，p-AKT(Thr-308)，GSK-3β and PI3K related nodes in the PI3K/AKT/GSK-3β pathway.Results:UVB acute light injury reduced ICAM-1 expression in the HaCat cell model and had no significant effect on the expressions of VCAM-1 and ELAM-1(P<0.01).The expressions of the three CAMs in the HaCat cell model were effectively inhibited in the G group(P<0.05)，while those of ELAM-1 and VCAM-1 were considerably impeded in the Z group(P<0.01).It was also found that acute UVB light injury had no significant effect on AKT phosphorylation and PI3K expression in the HaCat cell model.The low-concentration treatment of Haoqin Huaban Forumla can promote the phosphorylation of AKT in HaCat cells(P<0.05).Acute UVB light damage increased the GSK-3β expression level of HaCat cells(P<0.01).The high，medium and low concentration treatments of Haoqin Huaban Formula inhibited the GSK-3β expression level of HaCat cells(P<0.01).Conclusion:Haoqin-Huaban Formula can reduce the expression of adhesion molecules such as ICAM-1，ELAM-1 and VCAM-1 in HaCat cells caused by acute UVB damage，thereby alleviating the occurrence of local inflammation and delayed-type hypersensitivity，and can activate HaCat cells PI3K/AKT/GSK-3β pathway and plays a role in anti-oxidation，anti-apoptosis and reducing inflammation in the acute light damage caused by UVB. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
