世界中医药
文章摘要
引用本文:霍苏,崔鹤蓉,顾昱昊,戴子琦,李文,刘小靖,韩娜娜,马涛,王鹏龙,雷海民.基于网络药理学探究丹参治疗慢性肝炎的作用机制[J].世界中医药,2021,(20):.  
基于网络药理学探究丹参治疗慢性肝炎的作用机制
Action Mechanism of Radix Salviae Miltiorrhizae in the Treatment of Chronic Hepatitis Based on Network Pharmacology
投稿时间:2020-05-20  
DOI:10.3969/j.issn.1673-7202.2021.20.006
中文关键词:  网络药理学  丹参  慢性肝炎  药物作用机制  靶点  信号通路  生物功能
English Keywords:Network pharmacology  Radix Salviae Miltiorrhizae  Chronic hepatitis  Drug mechanism  Targets  Signaling pathway  Biological function
基金项目:中央高校基金科研业务项目(岐黄团队2019-JYB-TD005,杰出青年BUCM-2019-JCRC002和重点攻关2020-JYB-ZDGG-044);中华中医药学会青年人才托举工程项目(CACM-2018-QNRC2-B08)
作者单位
霍苏,崔鹤蓉,顾昱昊,戴子琦,李文,刘小靖,韩娜娜,马涛,王鹏龙,雷海民 北京中医药大学中药学院北京102488 
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中文摘要:
      目的:运用网络药理学的方法预测丹参治疗慢性肝炎的主要活性成分、靶点及信号通路,探究其潜在作用机制。方法:通过中药系统药理学数据库与分析平台(TCMSP)筛选丹参的有效成分;输入Pharm Mapper数据库获得相应基因;检索GeneCards数据库获得慢性肝炎的靶点基因;利用R语言3.6.3截取交集网络获得潜在靶点;使用Cytoscape3.7.2将“中药-化合物-靶点-疾病”网络可视化处理;在STRING平台构建蛋白质-蛋白质相互作用(PPI)网络;利用DAVID数据库对候选靶点进行基因本体(GO)富集分析和京都基因和基因组百科全书(KEGG)通路注释。结果:获得丹参中65个活性化合物、109个不重复的靶标信息、89个慢性肝炎治疗靶点。GO功能富集得到GO条目48个(P<0.05),其中生物过程(BP)条目1 796个,细胞组分(CC)条目73个,分子功能(MF)条目130个;KEGG通路富集得到88条信号通路(P<0.05)。结论:丹参中的有效成分异隐丹参酮(Isocryptotanshi-none)、1,2,5,6-tetrahydrotanshinone、丹参醌新酮Ⅰ(MiltiononeⅠ)、二氢丹参内酯(Dihydrotanshinlactone)、4-methylenemiltirone、丹参酮Ⅱa(TanshinoneⅡa)、木犀草素等可能作用于ADRB2、OPRM1、PTGS1、CA2、PTGS2等关键靶点通过调控Hepatitis B、IL-17 signaling pathway、Hepatitis C、T cell receptor signaling pathway、PI3K/AKT signaling pathway等干预了活性氧代谢过程、循环系统血管功能、氧化应激反应、凋亡,从而发挥治疗慢性肝炎的作用。
English Summary:
      To predict the main active components,targets and signal pathways of Radix Salviae Miltiorrhizae in the treatment of chronic hepatitis and to explore the mechanism of action.Methods:The effective components of Radix Salviae Miltiorrhizae were screened by TCMSP database.The corresponding genes were obtained by entering Pharm Mapper database.The target genes of chronic hepatitis were obtained by searching GeneCards database.We used R language 3.6.3 intercept intersection network to obtain candidate targets; With the help of Cytoscape 3.7.2,the network of “drug-compound-target-disease” was visualized.We built PPI network on STRING platform; the DAVID database was used for GO enrichment analysis and KEGG pathway annotation of the candidate targets.Results:A total of 65 effective compounds,109 non-repeated targets and 89 disease treatment targets were obtained.There were 48 GO items(P<0.05),among which 1796 were biological process(BP) items,73 were cell components(CC) items,and 130 were molecular function(MF) items.There were 88 signal pathways enriched by KEGG pathway(P<0.05).Conclusion:The effective ingredients of Radix Salviae Miltiorrhizae Isocryptotanshi-none,1,2,5,6-tetrahydrotanshinone,miltionone I,dihydrotanshinlactone,4-methylenemiltirone,Tanshinone IIa,Luteolin may act on key targets such as ADRB2,OPRM1,PTGS1,CA2,and PTGS2 by regulating Hepatitis B,IL-17 signaling pathway,Hepatitis C,T cell receptor signaling pathway,PI3K-AKT signaling pathway,etc.,intervening in the process of reactive oxygen metabolism,circulatory system vascular function,oxidative stress response,apoptosis,and thus play a role in the treatment of chronic hepatitis.
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