To predict the possible quality markers of Radix et Rhizoma Rhei in the prevention and treatment of prostate cancer based on integrated pharmacology and molecular docking techniques.Methods:Through the integrated pharmacology research platform V2.0(TCMIPV2.0) of traditional Chinese medicine,the chemical information of Radix et Rhizoma Rhei was predicted,and the protein interaction(PPI) network was constructed with the disease target information of prostate cancer,so as to carry out the enrichment analysis of the key targets of drug intervention.On this basis,we used the multi-dimensional network of chemical components,key targets,and disease pathways of Radix et Rhizoma Rhei,draw the network diagram of “component-target-pathway-disease” of Chinese medicinal,analyzed and obtained the main components acting on these key targets,and then base on the quality mark of traditional Chinese medicinal,the “Five Principles” of Q-marker combined with known related literature were used predict and analyze the quality markers of Radix et Rhizoma Rhei in preventing and treating prostate cancer.Finally,the AutoDock Vina molecular docking software was used to calculate and verify the molecular docking of candidate components and key targets.Results:A total of 14 active components of Radix et Rhizoma Rhei against prostate cancer,21 key core targets and 11 signal pathways were screened.Molecular docking showed that there was a good binding between AR and 8 components of Radix et Rhizoma Rhei anthraquinones which were all in the range of optimal oil-water partition coefficient.Conclusion:Based on the integration of pharmacological V2.0 platform and previous literature research,the possible quality markers of Radix et Rhizoma Rhei in the prevention and treatment of prostate cancer are Radix et Rhizoma Rhei anthraquinones,including emodin,Rhein,chrysophanol,aloe-emodin,emodin methyl ether,etc.,which have pharmacological effects on AR of the common target of drugs and diseases.