In this study,the anticancer protein(ASAP) of the larvae was isolated and purified,and the anticancer effects of the main components of the anticancer protein of the larvae were studied.Methods:We separated ASAP by anion exchange chromatography,hydrophobic interaction chromatography,and gel filtration chromatography; combined p53 gene expression activation and CCK-8 cell viability assay to track the anticancer activity of the separated components; the CCK-8 cell viability assay detected the effects of the main components of the anti-cancer protein of ASAP on the viability of human colorectal cancer Colo205,HT-29,human lung cancer A549,and human chronic myelogenous leukemia K562; Annexin-V/PI double staining combined with flow cytometry was used to detect cell apoptosis.Results:C6,the main component of the anticancer active protein isolated from ASAP,can inhibit the cell viability of Colo205,HT-29,A549,and K562 to varying degrees within 24 to 72 hours.Among them,it has the most inhibitory effect on Colo205 cells.Obviously,the IC50 was 5.7 μg/mL at 72 h; C6 can induce Colo205 cell apoptosis in a dose-dependent manner; C6 can inhibit the expression of anti-apoptotic proteins Mcl-1 and Bcl-2,and up-regulate the apoptotic protein Bax expression and cause the decrease of the apoptotic protein caspase-3 precursor procaspase-3.Conclusion:C6,the main component of the anti-cancer protein of ASAP,has an inhibitory effect on the viability of a variety of human cancer cells,and can induce caspase pathway-dependent apoptosis in human colorectal cancer Colo205 cells.