To investigate the effect of Tongluo Yishen Recipe（TLYSR） on renal interstitial fibrosis and ADM expression in UUO rats,and to explore its possible mechanism.Methods:A total of 50 male SD rats were randomly divided into 5 groups:a sham operation group,a model group,a TLYSR group,a valsartan group and a ADM treatment group,with 10 rats in each group.Corresponding intervention measures were taken in each group.The pathological changes of kidney were observed by Masson staining; the expression of E-cadherin in kidney was detected by IHC; the content of serum ADM was detected by radioimmunoassay; the content of serum PCⅢ and ADM in renal tissue were detected by ELISA,and the correlation between ADM and E-cadherin was analyzed.Results:Compared with sham operation group,the levels of serum ADM and PCⅢ in model group were significantly increased(P<0.01),and the levels of E-cadherin and ADM in renal tissue were decreased(P<0.01).Compared with the model group,the contents of serum ADM and PCⅢ in TLYSR group and valsartan group were decreased(P<0.01,P<0.05),and the contents of E-cadherin and ADM in renal tissue were increased(P<0.01,P<0.05); the contents of serum PCⅢ in ADM treatment group were decreased(P<0.05),and the contents of serum ADM,E-cadherin and ADM in renal tissue were increased(P<0.05),and the levels of ADM and E-cadherin in the renal tissue were positively correlated(r=0.62,P<0.01).Conclusion:TLYSR can improve renal interstitial fibrosis in UUO rats,inhibit the expression of fibrosis index PCⅢ and up regulate the expression of E-cadherin,which may be achieved by up regulating the expression of ADM protein in kidney.