To investigate the effects and mechanism of Allicin on apoptosis of Ishikawa cells in human endometrial carcinoma.Methods:Ishikawa cells in logarithmic growth phase was treated with DMSO(blank control group),Allicin(12.5,25,50 μg/mL) and LY294002 5 μg/mL.48 h later,the cell proliferation inhibition rate was detected by CCK-8 method,cell apoptosis was analyzed by flow cytometry,and protein expression of p-PI3K,p-AKT,Cleaved Caspase-3,Bcl-2,and Bax were detected by Western blotting.Results:Compared with the blank control group,intervention of Allicin 12.5,25,50 μg/mL and LY294002 5μg/mL could increase Ishikawa cells proliferation inhibition rate and apoptosis rate,down-regulate the expression of p-PI3K,p-AKT,Bcl-2 and up-regulate the expression of Cleaved Caspase-3,Bax,increase the ratio of Bax/Bcl-2,all of which were statistically significant(P<0.05 or P<0.01).Compared with the LY294002 5μg/mL group,intervented by Allicin 50 μg/mL could increase Ishikawa cells proliferation inhibition rate and apoptosis rate,down-regulate the expression of p-PI3K,p-AKT and up-regulate the expression of Caspase-3,Bax,increase the ratio of Bax/Bcl-2,all of which were statistically significant(P<0.05 or P<0.01).Conclusion:Allicin can induce apoptosis of human endometrial cancer Ishikawa cells,which may be related to the inhibition of PI3K/AKT signaling pathway activation and up-regulation of downstream pro-apoptotic proteins.