| 引用本文:陈晨1，李永圆1，王海霞1,2,3，于洋1,2,3，李正1,2,3.基于激光诱导击穿光谱技术的纳米银定性定量分析方法开发[J].世界中医药,2021,(23):. |
|
| 基于激光诱导击穿光谱技术的纳米银定性定量分析方法开发 |
| Development of Qualitative and Quantitative Analysis Method of Silver Nanoparticles Based on Laser Induced Breakdown Spectroscopy |
| 投稿时间：2021-10-25 |
| DOI：10.3969/j.issn.1673-7202.2021.23.004 |
| 中文关键词: 激光诱导击穿光谱 纳米银 定量分析 定性分析 |
| English Keywords:Laser induced breakdown spectroscopy Nano-silver particles Quantitative analysis Qualitative analysis |
| 基金项目:科技部“重大新药创制”科技重大专项(2018ZX09201011);国家自然科学基金项目(82003944) |
| 作者 | 单位 | | 陈晨1，李永圆1，王海霞1,2,3，于洋1,2,3，李正1,2,3 | 1 天津中医药大学中药制药工程学院,天津,301617
2 省部共建组分中药国家重点实验室,天津,301617
3 现代中医药海河实验室,天津,301617 |
|
| 摘要点击次数: 177 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的:纳米粒子特有的小尺寸效应和表面效应使得纳米银粒子具有广谱抗菌活性。但纳米银粒子浓度过高可能会对其他细胞或器官等造成毒性或损伤,所以为了更好地发挥抗菌活性,对纳米银粒子进行快速、准确的分析是极为必要的。方法:通过对纳米银粒子吸附方法和纸基芯片的优化和筛选,测定纳米银粒子的激光诱导击穿光谱图。通过特征峰位与标准谱图库进行比对对纳米银粒子进行定性分析,通过特征光谱强度与纳米银浓度的线性回归关系建立纳米银粒子分析的定量模型。结果:通过重复3次“浸渍-干燥”工艺,定量滤纸表面吸附的纳米银粒子在328 nm、338 nm、520 nm、546 nm和827 nm处显示出银的特征峰。在312.5~5 000 μg/mL浓度范围内,520 nm波长处纳米银的特征峰强与浓度呈良好线性关系(r=0.998 8)。结论:激光诱导击穿光谱分析技术可用于纳米银定性定量分析,分析方法准确,灵敏度高,且检测过程方便便捷,易于实际推广。 |
| English Summary: |
| The unique small size effect and surface effect of nanoparticles make silver nanoparticles have broad-spectrum antibacterial activity.However,too high concentration of silver nanoparticles may cause toxicity or damage to other cells or organs.Therefore,in order to better exert antibacterial activity,it is extremely necessary to analyze silver nanoparticles quickly and accurately.Methods:By optimizing and screening the nano-silver particle adsorption method and paper-based chip,the laser-induced breakdown spectra of nano-silver particles were measured.The qualitative analysis of nano-silver particles was carried out by comparing the characteristic peak position with the standard spectral library,and the quantitative model of nano-silver particle analysis was established by the linear regression relationship between the characteristic spectral intensity and the concentration of nano-silver.Results:By repeating the “dipping-drying” process three times,the nano-silver particles adsorbed on the surface of the quantitative filter paper showed characteristic peaks of silver at 328 nm,338 nm,520 nm,546 nm and 827 nm.In the concentration range of 312.5～5 000 μg/mL,the characteristic peak intensity of nano-silver at the wavelength of 520 nm had a good linear relationship with the concentration (r=0.998 8).Conclusion:The LIBS analysis technology can be used for qualitative and quantitative analysis of nano-silver.The analysis method is accurate and sensitive,and the detection process is convenient and convenient,which can be easily used in practice. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
