To investigate the mechanisms of Radix Astragali seu Hedysari in the treatment of viral myocarditis (VMC) based on a network pharmacology approach.Methods:Radix Astragali seu Hedysari'active ingredients were obtained from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP).The corresponding target proteins of active ingredients were obtained from TCMSP,and the protein names were corrected to gene symbols by using UniProt database.We obtained the related genes of VMC in GeneCards and OMIM database.The target genes of Radix Astragali seu Hedysari in the treatment of VMC were obtained by the intersection of the target genes of Radix Astragali seu Hedysari and the related genes of VMC.Using Cytoscape 3.8.0“Radix Astragali seu Hedysari-Active ingredient-Target gene-VMC” network was constructed.The PPI data were downloaded from STRING database and the core target genes were obtained through screening in R 4.0.0.R 4.0.0 was used to perform Go and KEGG pathway enrichment analysis on the target genes of Radix Astragali seu Hedysari in the treatment of VMC.Results:A total of 20 active ingredients and 191 target genes of Radix Astragali seu Hedysari were obtained while 192 related genes of VMC were obtained.A total of 34 target genes of Radix Astragali seu Hedysari in the treatment of VMC were obtained by intersection.The“Radix Astragali seu Hedysari-Active ingredient-Target gene-VMC” network was constructed.VEGFA,AKT1,IL10,IL6,MMP9,TNF,etc.may be the core target genes for Radix Astragali seu Hedysari in the treatment of VMC.The enrichment of GO is mainly concentrated in the cytokine receptor binding,regulation of cell-cell adhesion,response to lipopolysaccharide,response to molecule of bacterial origin,cytokine activity,etc..The enrichment of KEGG pathway was mainly concentrated in the AGE-RAGE signaling pathway in diabetic complications,IL-17 signaling pathway,Hepatitis B,Influenza A,Chagas disease (American trypanosomiasis),etc.Conclusion:This study preliminarily reveals the core target genes,biological process and signal pathway of Radix Astragali seu Hedysari in the treatment of VMC,which provides reference for further research. |