| 引用本文:路颖慧，康念欣，袁炎炎，李晴霞，谭鹏.黄连-吴茱萸不同比例配伍水提物对线虫的毒性效应评价[J].世界中医药,2021,(24):. |
|
| 黄连-吴茱萸不同比例配伍水提物对线虫的毒性效应评价 |
| Evaluation of the Toxic Effects of Different Proportions of Coptidis Rhizoma and Tetradium ruticarpum Extracts on Caenorhabditis Elegans |
| 投稿时间：2020-07-05 |
| DOI：10.3969/j.issn.1673-7202.2021.24.002 |
| 中文关键词: 黄连 吴茱萸 秀丽隐杆线虫 寿命 体长 运动行为 毒性 炮制减毒 |
| English Keywords:Coptidis Rhizoma Tetradium ruticarpum Caenorhabditis elegans Lifespan Body length Sports behavior Toxicity Concoct attenuation |
| 基金项目:国家中医药管理局中药标准化项目（ZYBZH-Y-HUB-20）；北京中医药大学青年教师项目（2017-JYB-JS-056） |
|
| 摘要点击次数: 249 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的：评价不同比例黄连-吴茱萸配伍提取物对秀丽隐杆线虫的毒性效应。方法：以秀丽隐杆线虫为模式生物，制备不同比例黄连-吴茱萸配伍水提物，测定各水提物对秀丽隐杆线虫寿命、体长及运动行为的影响。每组30条线虫观察存活率，每天在相同时间观察线虫数量，以最后一条线虫死亡时间作为最长寿命；每组40条线虫观察体长，用Image J软件测量死亡线虫虚拟体长；每组30条线虫观察头尾摆动和身体弯曲频率，测定1 min内线虫头部摆动的次数（从一侧摆向另一侧再摆回来）和20 s内身体弯曲的次数（认为线虫相对于身体的长轴移动一个正弦周期的波长为1次身体弯曲）。结果：与黄连组比较，随吴茱萸相对比例增加，秀丽隐杆线虫的死亡率显著降低（P<0.01）；体长显著减少（P<0.01）；对秀丽隐杆线虫的运动行为没有明显的抑制作用。结论：吴茱萸在一定程度上能够降低黄连对秀丽隐杆线虫的毒性，延长其寿命；在生长发育和神经方面影响较小，本研究为黄连和吴茱萸的配伍与炮制减毒研究提供了新的方法。 |
| English Summary: |
| To evaluate the toxic effects of different proportions of Coptidis Rhizoma and Tetradium ruticarpum extracts on Caenorhabditis elegans.Methods:Coptidis Rhizoma was used as model organism and preparing water extracts of Coptidis Rhizoma and Tetradium ruticarpum in different proportions。Assessing the effects of various water extracts on the survival rate，body length，head and tail swinging frequency and body bending frequency of C.elegans.The number of 30 C.elegans were observed and counted at the same time every day,and the death time of the last C.elegan was taken as the maximum life span.The body length of 40 C.elegans was measured by Image J software after C.elegans were killed in the oven and photographed.Counting the number of head and tail swinging frequency of 30 C.elegans in 1min(from one side to the other and back again) and the number of body bending frequency of 30 C.elegans in 20s(the movement of nematodes relative to one wavelength along the long axis of the nematode was taken as one body bending) to evaluate the effects on motor behavior of C.elegans.Results:Compared with the group of Coptidis Rhizoma，as the relative proportion of Tetradium ruticarpum increasing,the mortality rate of C elegans was significantly decreased(P<0.01).The body length was significantly reduced(P<0.01).In the aspect of locomotion behavior,the addition of Tetradium ruticarpum can't significantly reduces the frequency of head swing and body flexion of C.elegans.Conclusion:To a certain degree Tetradium ruticarpum can reduce the toxicity of Coptidis Rhizoma to C elegans,extending its life.In terms of growth and nerves，it has less affection.And the research provided a new method for the study of Coptidis Rhizoma and Tetradium ruticarpum in pairing and concoction. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
