To apply network pharmacology research of couple medicine Rhizoma Coptidis and Cortex Phellodendri the main active ingredients in the treatment of ulcerative colitis，prediction of active ingredients targets，establish of“medicine-the active ingredient-disease-targets”，further discusses the couple medicine Rhizoma Coptidis and Cortex Phellodendri potential mechanisms for the treatment of ulcerative colitis.Methods:Traditional Chinese Medicine Systems Pharmacology and Analysis Platform was used to screen the chemical constituents of the couple medicine Rhizoma Coptidis and Cortex Phellodendri.The relevant target genes of ulcerative colitis(UC) were obtained with the help of human Gene database，and the key action targets of the couple medicine Rhizoma Coptidis and Cortex Phellodendri in the treatment of ulcerative colitis were obtained by taking the intersection of drug action targets and disease-related target genes.The STRING database was used to construct protein interaction networks for key targets，and the results were visualized by using Cytoscape software.The DAVIDdatabase was used to analyze the functional and metabolic pathways of target genes related to the treatment of ulcerative colitis in the couple medicine Rhizoma Coptidis and Cortex Phellodendri.Results:A total of 34 key target proteins and 30 molecular signaling pathways for the couple medicine Rhizoma Coptidis and Cortex Phellodendri were screened.Main key genes such as PTGS2，IL6，CXCL8,and T cell receptor signaling pathway，NF-kappa B signaling pathway，NOD-like receptor signaling pathway and Toll-like receptor signaling pathway.Conclusion:The treatment of ulcerative colitis with the couple medicine Rhizoma Coptidis and Cortex Phellodendri is a complex process of multiple targets and multiple signaling pathways.The prediction of the potential mechanism provides theoretical basis for further study of the mechanism of action of the heat-clearing method in the treatment of ulcerative colitis.