To systematically evaluate the effect of acupuncture and moxibustion in the treatment of post-stroke patients with upper limb spastic hemiplegia.Methods:Six databases，including China National Knowledge Infrastructure(CNKI)，China Science Periodical Database(CSPD)，Chinese Citation Database(CCD)，China Biology Medicine disc(CBMdisc)，PubMed，and Cochrane，were searched for randomized controlled trials(RCTs) of acupuncture and moxibustion in the treatment of post-stroke patients with upper limb spastic hemiplegia published from database inception to May 1st，2020.Literature screening and data extraction were conducted by two independent researchers.The quality of the included research papers was evaluated by Cochrane risk-of-bias tool，and the data from the included papers underwent Meta-analysis by RevMan 5.3.Results:Twenty-five research papers(containing 31 sets of randomized controlled data) were included，with 1 963 patients involved.As revealed by the Meta-analysis results，the total effective rate of the observation group was higher than that of the control group，with OR=3.30，95%CI 1.97 to 5.52，Z=4.54，P<0.000 01; the post-treatment score of the Modified Ashworth Scale(MAS) of the observation group was lower than that of the control group，with SMD=-0.50，95%CI-0.90 to 0.11，Z=2.49，P=0.01; the Fugl-Meyer Assessment(FMA) score after treatment of the observation group was higher than that of the control group，with SMD=1.28，95%CI 0.89 to 1.67，Z=6.40，P<0.000 01; the Barthel Index(BI) after treatment of the observation group was higher than that of the control group，with SMD=1.12，95%CI 0.74 to 1.49，Z=5.87，P<0.000 01; the post-treatment score of the Clinic Neurological Function Deficit Scale(NDS of the observation group was lower than that of the control group，with WMD=-3.97，95%CI-6.22 to -1.72)，Z=3.46，P=0.000 5; the Brunnstrom score after treatment of the observation group was higher than that of the control group，with SMD=0.91，95%CI 0.53 to 1.29，Z=4.71，P<0.000 01; the Clinic Spasticity Index(CSI) after treatment of the observation group was lower than that of the control group，with SMD=-1.36，95%CI-2.76 to 0.03，Z=1.92，P=0.05.Conclusion:Acupuncture and moxibustion can effectively improve the spasm of the hemiplegic upper limb after stroke，with good safety and patient compliance.