| 引用本文:李琪1,吴梦蝶1,刘世敏2,萧有智3,王文佳1,马喆2,黄艳2,李灵杰1,2,李璟1,刘慧荣1,2.隔物灸调控缺氧诱导因子-1α表达抑制慢性萎缩性胃炎大鼠糖酵解的机制研究[J].世界中医药,2022,(03):. |
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| 隔物灸调控缺氧诱导因子-1α表达抑制慢性萎缩性胃炎大鼠糖酵解的机制研究 |
| Mechanism of Indirect Moxibustion in Inhibition of Glycolysis in Rats with Chronic Atrophic Gastritis by Regulating HIF-1α Expression |
| 投稿时间：2022-01-06 |
| DOI：10.3969/j.issn.1673-7202.2022.03.006 |
| 中文关键词: 艾灸疗法 隔药饼灸 隔姜灸 慢性萎缩性胃炎 缺氧诱导因子-1α 糖酵解 |
| English Keywords:Moxibustion Herb-partitioned moxibustion Ginger-partitioned moxibustion Chronic atrophic gastritis Hypoxia-inducible factor-1α Glycolysis |
| 基金项目:国家自然科学基金面上项目（81674071）；国家中医药领军人才支持计划“岐黄学者”；上海市卫生健康委员会科研课题计划资助（20214Y0115）；上海市重点专科（针灸）建设（shslczdzk04701） |
| 作者 | 单位 | | 李琪1,吴梦蝶1,刘世敏2,萧有智3,王文佳1,马喆2,黄艳2,李灵杰1,2,李璟1,刘慧荣1,2 | 1 上海中医药大学附属岳阳中西医结合医院，上海，200437； 2 上海市针灸经络研究所，上海，200030； 3 上海新起点康复医院，上海，201107 |
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| 中文摘要: |
| 目的：观察慢性萎缩性胃炎（CAG）大鼠胃黏膜组织缺氧诱导因子-1α（HIF-1α）及糖酵解关键酶表达变化，探究隔物灸对CAG的作用及可能机制。方法：将雄性Wistar大鼠随机分为正常组和造模组，自由饮用170 mg/L浓度MNNG联合法进行CAG大鼠造模。鉴定模型成功后，造模组大鼠随机分为模型组、隔药饼灸组、隔姜灸组和西药组，每组6只。隔药饼灸组、隔姜灸组均取中脘、气海穴进行隔物灸干预，西药组给予叶酸悬浊液灌胃。采用HE染色法观察胃窦组织病理学变化，实时荧光定量PCR（RT-qPCR)法检测胃黏膜组织信号转导及转录激活因子3（STAT3）、丙酮酸激酶M2（PKM2）和HIF-1α的mRNA表达水平，免疫组织化学法检测胃黏膜组织STAT3、HIF-1α蛋白表达，比色法和ELISA法测定胃黏膜组织LDH、PKM2活性。结果：与正常组比较，模型组大鼠胃黏膜组织固有腺体结构紊乱，腺体减少，出现萎缩、肠化、假幽门腺化生和（或）异型增生。模型组大鼠胃黏膜组织HIF-1α、STAT3、PKM2 mRNA表达上调（P<0.05），STAT3、HIF-1α蛋白表达增加（P<0.05），LDH、PKM2酶活性增加（P<0.05）。与模型组比较，隔药饼灸组、隔姜灸组、西药组固有腺体排列较规整，固有腺体萎缩、肠上皮化生、异型增生程度减轻；隔药饼灸和隔姜灸均能下调大鼠胃黏膜组织中HIF-1α、STAT3 mRNA与蛋白表达（P<0.05），降低LDH、PKM2酶活性（P<0.05）。结论：隔物灸中脘、气海穴可改善CAG大鼠胃窦组织形态学病变，修复胃黏膜损伤。抑制HIF-1α介导的异常糖酵解代谢水平可能是隔物灸法治疗CAG的效应机制之一。 |
| English Summary: |
| To observe the expression changes of HIF-1α and glycolysis enzymes in gastric mucosa of rats with chronic atrophic gastritis (CAG) and explore the mechanism of indirect moxibustion on CAG.Methods:Male Wistar rats were randomly divided into a normal group and an experimental group.The CAG model was induced in the experimental group by administration of MNNG （170 mg/L）.The model rats were then randomly divided into a model group，a herb-partitioned moxibustion (HM) group，a ginger-partitioned moxibustion (GM) group，and a western medicine (WM) group，with six rats in each group.“Zhongwan” (CV 12) and “Qihai” (CV 6) were selected for indirect moxibustion in the HM group and the GM group.The folic acid suspension was administered to the rats in the WM group by gavage.The histopathological changes of gastric antrum were observed by HE staining.The mRNA expression of STAT3，PKM2 and HIF-1α in the gastric mucosa was detected by RT-qPCR，and the protein expression of STAT3 and HIF-1α was detected by immunohistochemistry.The activities of LDH and PKM2 were determined by colorimetry and ELISA.Results:Compared with the normal group，the model group showed the structural disorder of inherent glands，reduced glands，atrophy，intestinal metaplasia，pseudopyloric metaplasia，and/or dysplasia in the gastric mucosa.Furthermore，the mRNA expression of HIF-1α，STAT3，and PKM2 in the gastric mucosa was up-regulated (P<0.05)，the protein expression of STAT3 and HIF-1α was elevated (P<0.05)，and the enzyme activities of LDH and PKM2 were potentiated in the model group (P<0.05).Compared with the model group，the HM group，the GM group，and the WM group showed the regular arrangement of inherent glands，and relieved inherent gland atrophy，intestinal metaplasia，and dysplasia.Compared with the model group，the HM group and the GM group displayed down-regulated mRNA and protein expression levels of HIF-1α and STAT3 in the gastric mucosa (P<0.05)，and weakened enzyme activities of LDH and PKM2 (P<0.05).Conclusion:Indirect moxibustion at “Zhongwan” (CV 12) and “Qihai” (CV 6) can improve the histological changes of the gastric antrum and repair gastric mucosa damage in rats with CAG，and the mechanism of indirect moxibustion in the treatment of CAG may be related to the inhibition of abnormal glycolysis mediated by HIF-1α. |
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