| 引用本文:吴璐一1,2,钟蕊3,林亚莹2,郑寒丹2,刘世敏2,黄艳2,马喆2,刘雅楠2.艾灸调节溃疡性结肠炎小鼠E3泛素连接酶TRIM31的肠黏膜屏障保护机制[J].世界中医药,2022,(03):. |
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| 艾灸调节溃疡性结肠炎小鼠E3泛素连接酶TRIM31的肠黏膜屏障保护机制 |
| Protective Mechanism of Moxibustion on Intestinal Mucosal Barrier by Regulating E3 Ubiquitin Ligase TRIM31 in Mice with Ulcerative Colitis |
| 投稿时间：2022-01-06 |
| DOI：10.3969/j.issn.1673-7202.2022.03.007 |
| 中文关键词: 溃疡性结肠炎 艾灸 TRIM31 肠黏膜屏障 炎症介质 基因敲除 |
| English Keywords:Ulcerative colitis Moxibustion TRIM31 Intestinal mucosal barrier Inflammatory factors Gene knockout |
| 基金项目:国家自然科学基金项目（81973955，82004475）；上海市卫生计生系统优秀人才培养计划-优秀青年人才（2018YQ11）；上海市卫生健康委员会科研课题计划资助（20194Y0005）；上海市重点专科（针灸）建设（shslczdzk04701） |
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| 中文摘要: |
| 目的：观察艾灸对野生型和TRIM31基因敲除（TRIM31-/-）溃疡性结肠炎（UC）模型小鼠紧密连接蛋白以及血清炎症介质的影响，探讨TRIM31能否通过保护UC肠黏膜屏障而降低UC肠道炎症反应以及艾灸对其调节作用。方法：分别采用野生型和TRIM31基因敲除小鼠构建UC模型，免疫组织化学检测结肠组织中TRIM31、闭合蛋白、密封蛋白、ZO-1的蛋白表达；实时荧光定量PCR（RT-qPCR)检测法检测结肠组织中TRIM31的mRNA表达；ELISA检测血清白细胞介素-1β（IL-1β）、IL-13、IL-25、IL-33蛋白的浓度。结果：艾灸可改善野生型UC小鼠的结肠炎症，升高结肠TRIM31蛋白及mRNA的表达以及肠黏膜屏障相关闭合蛋白、密封蛋白、ZO-1的表达，降低血清炎症介质IL-1β、IL-13、IL-25、IL-33的浓度；TRIM31-/-小鼠较野生型小鼠相比，结肠炎症加重，肠黏膜屏障相关闭合蛋白、密封蛋白、ZO-1表达降低，血清炎症介质IL-1β、IL-13浓度升高；艾灸还可改善TRIM31-/-UC小鼠的结肠炎症，升高结肠组织肠黏膜屏障相关闭合蛋白、密封蛋白、ZO-1的表达，降低血清炎症介质IL-1β、IL-13、IL-25、IL-33的浓度。结论：TRIM31蛋白可能是艾灸参与调节UC肠黏膜屏障保护的关键靶点；艾灸对TRIM31-/-小鼠肠黏膜屏障相关闭合蛋白、密封蛋白、ZO-1及血清炎症介质IL-1β、IL-13、IL-25、IL-33仍有调节作用，表明艾灸还可能通过发挥其多靶点调节作用参与UC肠黏膜屏障保护。 |
| English Summary: |
| To observe the effects of moxibustion on tight junction proteins and serum inflammatory factors in wild-type and TRIM31 knockout (TRIM31-/-) ulcerative colitis (UC) model mice and explore the possibility of TRIM31 in reducing intestinal inflammation in UC by protecting the intestinal mucosal barrier，as well as the regulatory role of moxibustion.Methods:The UC model was induced in the wild-type and TRIM31-/-mice.Immunohistochemistry was used to detect the protein expression of TRIM31，Occludin，Claudin-1，and ZO-1 in colon tissues.RT-qPCR was used to detect the mRNA expression of TRIM31 in colon tissues.The serum levels of IL-1β，IL-13，IL-25，and IL-33 were detected by ELISA.Results:Moxibustion could resist colonic inflammation in wild-type UC mice，increase the protein and mRNA expression of TRIM31 and the expression of Occludin，Claudin-1，and ZO-1 in the colon，and reduce the concentrations of inflammatory factors IL-1β，IL-13，IL-25，and IL-33 in the serum.Compared with the wild-type mice，the TRIM31-/-mice showed aggravated colitis，decreased expression of Occludin，Claudin-1，and ZO-1，and increased serum concentrations of IL-1β and IL-13.Additionally，moxibustion could improve the colitis of TRIM31-/-UC mice，increase the expression of Occludin，Claudin-1，and ZO-1 in the colon，and reduce the serum concentrations of IL-1β，IL-13，IL-25，and IL-33.Conclusion:TRIM31 protein might serve as a key target of moxibustion in regulating the intestinal mucosal barrier in UC.The regulatory role of moxibustion on Occludin，Claudin-1，ZO-1，IL-1β，IL-13，IL-25，and IL-33 in TRIM31-/-mice was verified，which suggested that moxibustion presumedly participated in the protection of intestinal mucosal barrier in UC by virtue of its multi-target regulatory role. |
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