Isolation and identification of chemical composition from the n-butanol fraction of Flos Daturae.Methods:Silica gel column chromatography，ODS column chromatography，silica gel thin layer chromatography and HPLC were used for separation and purification.The structure was identified by 1H-NMR，13C-NMR，MS and other spectral data.Results:A total of 11 compounds were extracted from the crude extract of Flos Daturae.They were identified as：Alkaloids：(S)-(-)-2-phenylpropionitrile(1)，merobatzelladine A(2)，magnof-lorine(3)，crotonine(4)，cyclo（Phe-Leu)(5)，pyrrolezanthine(6)，(E,E)-N,N-dityramin-4,4'-dihyd-roxy-3,5'-dimethoxy-β,3'-bicinnamamide(7)，N1,N5,N10 tri-p-coumaroylspermidine(8)，7-hy-dro-xy-1-(4-hydroxy-3-methoxyphenyl)-N2,N3-bis(4-hydroxyphenethyl)-6-methoxy-1,2-dihy-dronaphthalene-2,3-dicarboxamide(9).Other compounds：p-hydroxybenzoyl p-coumaric acid anhydri-de(10)，methyl-(Z)-9-octadecenoate(11).Conclusion:The above 11 compounds were isolated from Solanaceae for the first time.