To explore the mechanism of Zhisou Powder in the treatment of laryngeal cough by network pharmacology.Methods:Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP) was used to screen and mine the main components and targets of Zhisou Powder.The network diagram of traditional Chinese medicine components and targets was constructed via Cytoscape 3.7.2 software.The related targets of laryngogeal cough were mined via GeneCards platform.The Protein-protein Interaction(PPI) network diagram of Zhisou Powder in the treatment of laryngogeal cough was constructed via STRING database.Topology analysis was carried out and the therapeutic effect of Zhisou Powder was screened.David database was used to enrich and analyze the intersection targets of drugs and diseases.Results:The drug component target disease network diagram of Zhisou Powder contained 152 active components，155 corresponding targets，and 1 176 related targets of laryngeal cough.Among them，β-sitosterol，quercetin，luteolin，stigmasterol and kaempferol may be the key components of Zhisou Powder in the treatment of laryngeal cough，involving 14 core targets such as TP53，VEGFA，IL6，MAPK，Jun，TNF and EGF.The biological process of Gene Ontology(GO) analysis involves the positive regulation of nitric oxide biosynthesis，extracellular space，gene expression，extracellular domain and lipopolysaccharide mediated signaling pathway.Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis suggested that these targets were involved in TNF signaling pathway，nod like receptor signaling pathway，FoxO signaling pathway，Toll-like receptor signaling pathway，HIF-1 signaling pathway and so on.Conclusion:This study reveals the network regulation mechanism of Zhisou Powder in the treatment of laryngeal cough with multi-component，multi-target and multi-channel，and expounds the effective mechanism of Zhisou Powder in the treatment of laryngeal cough as a whole，so as to provide molecular thinking for the next experimental research.