| 引用本文:华雯，高鸿彬.基于网络药理学探讨三棱-莪术药对治疗肝癌的作用机制[J].世界中医药,2022,(05):. |
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| 基于网络药理学探讨三棱-莪术药对治疗肝癌的作用机制 |
| Discussion on the Mechanism of Action of Rhizoma Sparganii-rhizoma Curcumae Drug Pair on the Treatment of Liver Cancer Based on Network Pharmacology |
| 投稿时间：2020-09-17 |
| DOI：10.3969/j.issn.1673-7202.2022.05.015 |
| 中文关键词: 三棱-莪术药对 肝癌 网络药理学 中药 成分 靶点 通路 作用机制 |
| English Keywords:Rhizoma Sparganii-Rhizoma Curcumae drug pair Liver cancer Network pharmacology Chinese medicinal Composition Target Pathway Mechanism of action |
| 基金项目:军队医疗机构制剂标准提高计划科研专项课题（14ZJZ02-3）；上海交通大学医学院附属仁济医院宝山分院临床科研创新培育基金项目（2019-rbcxjj-005） |
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| 中文摘要: |
| 目的：采用网络药理学方法筛选三棱-莪术药对治疗肝癌的作用靶点以及相关信号通路，明确治疗肝癌的作用机制。方法：通过中药系统药理学数据库与分析平台（TCMSP）筛选出三棱、莪术的有效药物成分及作用靶点，构建有效成分-靶点蛋白质-蛋白质相互作用（PPI）网络。从OMIM、DrugBank、TTD数据库检索与肝癌相关的疾病靶点，并通过R语言软件分析获得三棱-莪术成分与肝癌的交集靶点。应用Cytoscape 3.2.1软件绘制成分-靶点网络，并运用DAVID数据库进行基因本体（GO）富集分析和京都基因和基因组百科全书（KEGG）通路富集分析。结果：共筛选出三棱-莪术药对与肝癌的相对应作用靶点共31个。共涉及与肝癌相关的GO分析125条，主要包括分子功能、生物过程、细胞组分3个方面。KEGG通路82条，主要从一氧化氮生物合成过程的正调控、细胞对脂多糖的反应、RNA聚合酶Ⅱ启动子转录的正调控等多处通路治疗肝癌。结论：本研究初步验证了三棱-莪术药对通过肿瘤坏死因子信号通路、RNA聚合酶Ⅱ启动子转录的正调控、类固醇激素介导的信号通路等治疗肝癌。本研究表明三棱-莪术药对具有多成分、多靶点的特点，其作用机制多样，对进一步研究肝癌的治疗提供了参考。 |
| English Summary: |
| To screen out the action targets and related signal pathways of Rhizoma Sparganii-Rhizoma Curcumae drug pair on the treatment of liver cancer by means of network pharmacology，and to clarify the mechanism of action in the treatment of liver cancer.Methods:The effective ingredients and targets of Rhizoma Sparganii-Rhizoma Curcumae drug pair were screened out through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)，and the effective ingredient-target Protein-protein Interaction(PPI) network was constructed.We retrieved disease targets related to liver cancer from OMIM，DrugBank，and TTD databases，and used R language software analysis to obtain the intersection target of the Rhizoma Sparganii-Rhizoma Curcumae drug pair component and liver cancer.Cytoscape 3.2.1 software was used to draw the component-target network，and the DAVID database was used for Gene Ontology(GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway analysis.Results:A total of 31 targets of Rhizoma Sparganii-Rhizoma Curcumae drug pair turmeric medicine against liver cancer were screened.A total of 125 GO analyses related to liver cancer were involved，including 3 aspects:molecular function，biological process，and cell composition.There were 82 KEGG pathways，mainly from the positive regulation of nitric oxide biosynthesis，the cell's response to lipopolysaccharide，and the positive regulation of RNA polymerase Ⅱ promoter transcription to treat liver cancer.Conclusion:This study preliminarily verified the treatment of liver cancer by Rhizoma Sparganii-Rhizoma Curcumae drug pair through the tumor necrosis factor signaling pathway，the positive regulation of RNA polymerase Ⅱ promoter transcription，and the steroid hormone-mediated signaling pathway.This study shows that the Rhizoma Sparganii-Rhizoma Curcumae drug pair has the characteristics of multiple components and multiple targets，and its mechanism of action is diverse，which provides a reference for further research on the treatment of liver cancer. |
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