To preliminarily study the therapeutic material basis of Xinshubao tablet in “promoting blood circulation and removing stasis” by a combined method of the drug-containing intestinal absorption liquid and the pharmacological activity evaluation in vitro.Methods:The intestinal absorption liquids containing Xinshubao prescription and five single ingredients were prepared，and the pharmacological effect of “promoting blood circulation and removing stasis” was reflected by the anticoagulant and antioxidant to find the single ingredient with the optimum efficacy.Four coagulation tests and adenosine diphosphate(ADP)-induced platelet aggregation test were conducted to detect the anticoagulant effect，and 1，1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging test was conducted to reveal the antioxidant effect.Results:The results of anticoagulant experiments showed that compared with the blank control group，Crataegi Fructus prolonged prothrombin time(PT)(P<0.01) and thrombin time(TT)(P<0.05)，and had a stronger effect on anti-platelet aggregation induced by ADP(P<0.01).The results of antioxidant experiments showed that compared with the blank control group，Salvia Root had the optimum effect on scavenging DPPH radical(P<0.01)，followed by Crataegi Fructus，Acanthopanacis，Senticosi Radix Et Rhizoma Seu Caulis，Paeoniae Radix Alba，and Curcumae Radix.Conclusion:Salviae Miltiorrhizae Radix Et Rhizoma and Crataegi Fructus may be the most important ingredients of Xinshubao tablet to exert the pharmacological effect of “promoting blood circulation and removing stasis”，but they are unable to represent the whole effect of Xinshubao tablet.