| 引用本文:曹一鸣，王悦竹，史亚婷，宫玮珩，刘雁峰.基于网络药理学探讨固本止崩汤对功能失调性子宫出血的机制研究[J].世界中医药,2022,(07):. |
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| 基于网络药理学探讨固本止崩汤对功能失调性子宫出血的机制研究 |
| Study on the Mechanism of Guben Zhibeng Decoction in the Treatment of Dysfunctional Uterine Bleeding Based on Network Pharmacology |
| 投稿时间：2020-08-24 |
| DOI：10.3969/j.issn.1673-7202.2022.07.009 |
| 中文关键词: 网络药理学 中医 固本止崩汤 功能失调性子宫出血 靶点 分子机制 信号通路 富集分析 |
| English Keywords:Network pharmacology Traditional Chinese Medicine Guben Zhibentang Dysfunctional uterine bleeding Target Molecular mechanism Signal pathway Enrichment analysis |
| 基金项目:国家自然科学基金面上项目(81473721);首都卫生发展科研专项(首发2020-2-4194) |
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| 中文摘要: |
| 目的:运用网络药理学的方法探讨固本止崩汤对功能失调性子宫出血的可能性机制。方法:在中药系统药理学数据库与分析平台筛选固本止崩汤的活性成分并预测其潜在作用靶点,将获得靶点导入UNIPROT数据库进行基因的标准化处理;在GeneCards和OMIM数据库查找功能失调性子宫出血的相关靶点。运用Cytoscape 3.7.2软件构建“药物-成分-靶点-疾病”网络图,将二者交集靶点运用STRING平台分析蛋白间的相互作用关系。用DAVID数据库对交集靶点进行基因本体（GO）生物功能和富集分析和京都基因和基因组百科全书（KEGG）通路富集分析,并以图表形式展示。结果:以口服生物利用度(OB)和类药性(DL)2个筛选标准得到固本止崩汤中有效活性成分58个,得到靶点135个;在GeneCards和OMIM查找功能失调性子宫出血靶点共2 164个,韦恩图获得药物与疾病的交集靶点共85个,关键靶点涉及IL6、VEGFA、TP53、TNF、EGF等。以P富集分析得到GO条目135条,其中生物过程38条、分子功能36条、细胞组分61条,主要涉及对缺氧的反应、一氧化氮生物过程的正向调节、细胞外空间、细胞外区域、酶结合、类固醇结合等;KEGG富集分析得到25条通路(P<0.05,FDR＜0,05),靶基因主要富集在癌症信号通路等信号通路中。结论:固本止崩汤治疗功能失调性子宫出血具有多成分、多靶点、多通路的特点,其作用机制较为复杂,为后续的实验验证提供物质基础和理论依据。 |
| English Summary: |
| To explore the possible molecular mechanism of Guben Zhibeng Decoction in the treatment of dysfunctional uterine bleeding by means of network pharmacology.Methods:The active components of Guben Zhibeng Decoction were screened on the platform of pharmacology database of traditional Chinese medicine system and their potential action targets were predicted,and then the obtained targets were imported into UNIPROT database for gene standardization treatment; the related targets of dysfunctional uterine bleeding was searched in GeneCards and OMIM databases.The network diagram of “drug-component-target-disease” was constructed by using Cytoscape 3.7.2 software,and the interaction between proteins was analyzed by using STRING online platform.GO biological function and KEGG pathway enrichment were analyzed by DAVID database,and the results were displayed in graph form.Results:A total of 58 effective active ingredients in Guben Zhibeng Decoction were obtained by OB and DL screening criteria,and 135 targets were predicted.A total of 2 164 targets of dysfunctional uterine bleeding were found in GeneCards and OMIM,and 85 targets of intersection of drugs and diseases were found in Venn diagram.The key targets involved IL6,VEGFA,TP53,TNF,EGF,etc.A total of 135 GO items were obtained by P enrichment analysis,including 38 biological processes,36 molecular functions and 61 cellular components,mainly involving in hypoxia response,positive regulation of nitric oxide biosynthetic process,extracellular space,extracellular region,enzyme binding,steroid binding,etc.A total of 25 pathways were found by KEGG enrichment analysis(P<0.05,FDR<0.05),and the target genes were mainly enriched in cancer signal pathway etc.Conclusion:Guben Zhibeng Decoction has the characteristics of multi-component,multi-target and multi-channel in the treatment of dysfunctional uterine bleeding,and its mechanism is complex,which provides material basis and theoretical basis for subsequent experimental verification. |
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