To study the mechanism of Sanzi Yangqin Decoction in the treatment of allergic asthma.Methods:Gene Expression Omnibus 2R(GEO2R) was used to find the differential gene of GSE40889 chip，and Gene-Cloud Biotechnology Information(GCBI) database was used to make the differential gene heat map.DAVID 6.8 was used to conduct Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analyses on the differential gene.Cytoscape was used to construct the network of protein-protein interaction.The core gene was screened out，and the network was analyzed.The module analysis of the differential gene was carried out by the Molecular Complex Detection(MCODE) of Cytoscape.Results:A total of 21 active components were queried，268 corresponding targets were found.A total of 213 differential genes were screened out，which were mainly enriched in membrane tissue，ribonucleic acid(RNA) binding，transcription，and translation，involving signaling pathways of tumor necrosis factor-α(TNF-α)，B cell receptor，transforming growth factor-β(TGF-β)，and phosphoinositide 3 kinase/protein kinase B(PI3K/AKT).An important module was obtained，which mainly focused on the regulation of gene silencing，protein isomerization，cell-cell adhesion，virus process，the positive regulation of RNA polymerase II promoter transcription，the response of endogenous apoptosis signaling pathway mediated by tumor suppressor p53 to deoxyribonucleic acid(DNA) damage，DNA template，and the positive regulation of host to virus transcription，involving signaling pathways of Notch，forkhead box O(FoxO)，and PI3K-AKT.Conclusion:Sanzi Yangqin decoction may regulate gene silencing，make protein isomerization，and affect transcription and translation through the signaling pathways of TNF-α，B cell receptor，TGF-β，and PI3K/AKT.