Based on the concept of Q-marker，high performance liquid chromatography(HPLC) fingerprints were established for Radix Scutellariae and the content of two components was determined.Via network pharmacology，the potential targets of the two components were predicted for determining the Q-markers of the medicinal.Methods:The HPLC fingerprints of 10 batches of Radix Scutellariae were established，and the content of baicalin and wogonoside was determined.Based on network pharmacology，the targets of the active components and related pathways were analyzed and the “component-target-pathway” network was plotted.Thereby，pharmacological characteristics of Radix Scutellariae were clarified and the potential Q-markers were forecasted.Results:The fingerprints of Radix Scutellariae shared 12 peaks.Two of them were identified，namely，baicalin and wogonoside，and the content was determined.The fingerprints of the 10 batches of samples showed>0.970 similarity to the reference fingerprint.A total of 8 key targets which were involved in 9 pathways such as inflammation pathway，cancer pathway，and immune pathway，were screened out.Conclusion:The HPLC method is accurate and reliable，and two components can be used as potential Q-markers of Radix Scutellariae.The mechanism related to the principal components of Radix Scutellariae was further tested by network pharmacology，which confirmed that baicalin and wogonoside can be used as Q-markers of the medicinal.The result is expected to serve as a reference for quality control of Radix Scutellariae.