| 引用本文:林家茂1，张晨月2，部帅3，李振祥1，赵成龙1，王海永1.功能基因组学方法筛选蟾毒灵抗肝癌细胞的潜在凋亡靶点[J].世界中医药,2022,(08):. |
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| 功能基因组学方法筛选蟾毒灵抗肝癌细胞的潜在凋亡靶点 |
| Screening of Potential Targets of Bufalin against Hepatocellular Carcinoma Cells by Functional Genomics |
| 投稿时间：2021-03-11 |
| DOI：10.3969/j.issn.1673-7202.2022.08.002 |
| 中文关键词: 蟾毒灵 肝癌 增殖 凋亡 转录组学 体外 基因表达 生物信息学 |
| English Keywords:Bufalin Hepatocellular carcinoma Proliferation Apoptosis Transcriptome In vitro Gene expression Bioinformatics |
| 基金项目:山东省重点研发计划项目（2018GSF119014）；山东省泰山学者基金资助项目（tsqn201812149）；山东第一医科大学学术提升计划项目（2019RC004） |
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| 中文摘要: |
| 目的：筛选中药单体蟾毒灵促进人肝癌细胞凋亡的潜在分子靶点。方法：以人肝癌细胞株PLC/PRF/5为体外模型，观察中药单体蟾毒灵对人肝癌细胞凋亡以及相关基因表达的影响。以不同浓度的蟾毒灵分别作用于PLC/PRF/5细胞24 h、48 h、72 h，检测其对人肝癌细胞增殖和凋亡的影响。通过高通量Illumina RNA测序，筛选蟾毒灵作用人肝癌细胞后基因表达的变化，并进行生物信息学分析。结果：中药单体蟾毒灵可抑制人肝癌细胞增殖并诱导其凋亡；中药单体蟾毒灵可诱导不同基因的表达；基因本体（GO）富集分析表明，中药单体蟾毒灵可以影响细胞周期、凋亡等通路的变化；京都基因和基因组百科全书（KEGG）富集分析凋亡通路表明，钙离子诱导的细胞死亡途径中的钙蛋白酶表达明显上调。结论：中药单体蟾毒灵具有明显的抗肝癌细胞凋亡作用，并可通过作用相关信号通路中的多靶点参与此过程。 |
| English Summary: |
| This study aims to screen molecular targets of bufalin in promoting apoptosis of human hepatocellular carcinoma cells.Methods:The effect of bufalin on apoptosis and gene expression of human hepatocellular carcinoma PLC/PRF/5 cells was investigated.To be specific，the PLC/PRF/5 cells were treated with different concentration of bufalin for 24 h，48 h，and 72 h，respectively，and the proliferation and apoptosis of the cells were detected.Illumina high-throughput sequencing of RNA was performed to observe the changes of gene expression in the cells treated with bufalin，and the bioinformation of related genes was analyzed.Results:Bufalin inhibited the proliferation and induced the apoptosis of PLC/PRF/5 cells.It induced the expression of different genes.Gene Ontology(GO) term analysis showed that bufalin influenced cell cycle，apoptosis，and other pathways.Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway analysis suggested that calpain was significantly up-regulated in calcium-induced cell death pathway.Conclusion:Bufalin remarkably promotes the apoptosis of human hepatocellular carcinoma cells through multiple targets in related pathways. |
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