Abstract:To study the effects of different concentrations of Kanglaite Injection(KLT) on the proliferation and apoptosis of human Burkitt's lymphoma Raji cells,and to explore the mechanism of KLT in preventing and treating malignant tumors.Methods:Flow cytometry and CCK-8 assay were employed to study the effects of KLT on the growth of Raji cells at the time points of 12,24,and 48 h.Results:The results of flow cytometry showed that the exposure to 1,10,50,100,and 200 μL/mL KLT for 12 h caused the apoptosis rates of -0.95%,50.93%,100%,44.39%,and -9.94%,respectively,in Raji cells.The CCK-8 assay demonstrated that 1,10,and 50 μL/mL KLT treatments for 12 h showed the inhibition rates of 55.41%,25.15%,and 39.94%,respectively,on Raji cells,while 100 μL/mL and 200 μL/mL KLT led to the cell proliferation rates of 122.62% and 146.81%,respectively.The 1,10,50,100,and 200 μL/mL KLT treatments for 24 h led to the inhibition rates of 32.25%,26.24%,31.61%,129.49%,and 160.09%,respectively.The treatments for 48 h caused the inhibition rates of 91.65%,81.47%,33.74%,103.75%,and 50.97%,respectively.Conclusion:KLT has dual regulatory effects on the growth of Raji cells,which is associated with the treatment time and KLT concentration.