To construct a component-target-pathway network and decipher the mechanism of Yinzhihuang Oral Liquid in the treatment of jaundice based on network pharmacology.Methods:The active components of the four herbal medicines(Artemisiae Scopariae Herba,Gardeniae Fructus,Scutellariae Radix,and Lonicerae Japonicae Flos) in Yinzhihuang Oral Liquid were obtained from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),on the basis of which the potential targets were predicted.Venn diagram and component-target network were established.The jaundice-related targets were obtained from OMIM,GeneCards,and Drugbank.The common targets shared by the active components and jaundice were imported into STRING and Cytoscape 3.7.2 to establish the protein-protein interaction(PPI) network.Metascape was employed to perform gene ontology(GO) annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment of the key targets.Results:A total of 530 chemical components of Yinzhihuang Oral Liquid were retrieved from TCMSP.According to the oral bioavailability(OB) and drug likeness(DL),87 active components were screened out.A total of 233 targets associated with jaundice were screened out.The active components and jaundice shared 122 common targets.The enriched GO terms indicated that the common targets were mainly involved in the regulation of cell apoptosis,cytokine-receptor binding,and the activities of protein kinases.The KEGG pathway enrichment predicted that the targets mainly participated in the T cell receptor,IL-17,PI3K-AKT,and NF-κB signaling pathways.Conclusion:Yinzhihuang Oral Liquid treats jaundice via multiple components and multiple targets.The possible mechanism of action predicted in this study provides a theoretical basis for later experimental research.