| 引用本文:刘畅，张慧月，聂晶，刘丽慧，张荻，吴记勇.基于网络药理学的鼻渊通窍颗粒治疗过敏性鼻炎的分子机制研究[J].世界中医药,2022,(12):. |
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| 基于网络药理学的鼻渊通窍颗粒治疗过敏性鼻炎的分子机制研究 |
| Molecular Mechanism on Biyuan Tongqiao Granules in Treatment of Allergic Rhinitis Based on Network Pharmacology |
| 投稿时间：2021-12-03 |
| DOI：10.3969/j.issn.1673-7202.2022.12.003 |
| 中文关键词: 鼻渊通窍颗粒 过敏性鼻炎 网络药理学 分子机制 靶点 |
| English Keywords:Biyuan Tongqiao Granules Allergic rhinitis Network pharmacology Molecular mechanism Target |
| 基金项目:国家自然科学基金青年科学基金项目（81903490） |
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| 中文摘要: |
| 目的：通过网络药理学方法筛选鼻渊通窍颗粒化学成分，构建成分-疾病靶点网络，研究其治疗过敏性鼻炎的潜在分子机制。方法：通过中药系统药理学数据库与分析平台（TCMSP）和DrugBank数据库，筛选鼻渊通窍颗粒所含的化学成分，并获取其作用靶点；应用GeneCards数据库，以“Allergic Rhinitis”为关键词检索过敏性鼻炎疾病靶点；获得药物和疾病的共同靶点并导入STRING数据库得到蛋白质-蛋白质相互作用（PPI）关系，通过Cytoscape 3.7.2构建PPI网络。使用clusterProfiler进行基因本体（GO）富集分析和京都基因和基因组百科全书（KEGG）富集分析。以药物网络分子中结合位点较多的槲皮素、芹菜素、山柰酚通过AutoDock软件同PTGS1、PTGS2，以及与过敏性鼻炎密切相关的血清因子IgE抗体进行分子对接。结果：网络药理学研究发现鼻渊通窍颗粒治疗过敏性鼻炎可能的活性成分为槲皮素、芹菜素、山柰酚、熊果酸、迷迭香酸，通过调节PTGS2、PTGS1、CHRM2、ADRB2、NOS2等靶点，调控MAPK、HIF-1、核因子κB、cAMP等信号通路，发挥其抑制炎症的作用。分子对接结果显示，关键成分对PTGS1、PTGS2和IgE抗体具有良好的亲和性。结论：本研究基于网络药理学理论和分子对接技术揭示了鼻渊通窍颗粒以多成分、多靶点、系统调节过敏性鼻炎，为临床药物治疗提供依据。 |
| English Summary: |
| To screen the chemical components of Biyuan Tongqiao Granules and construct an “active component-disease target” network by network pharmacology to explore the underlying mechanism of Biyuan Tongqiao Granules in the treatment of allergic rhinitis(AR).Methods:The chemical components of Biyuan Tongqiao Granules were screened out from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP) and DrugBank，and their targets were obtained.The AR-related targets were searched from GeneCards with the term “allergic rhinitis”.The common targets of the drug and the disease were obtained and imported into the STRING to obtain the protein-protein interaction(PPI) network which was plotted by Cytoscape 3.7.2.Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analyses were performed using clusterProfiler.Quercetin，apigenin，and kaempferol with abundant binding sites in drug network molecules underwent molecular docking with PTGS1，PTGS2，and serum factor IgE antibodies closely related to AR through AutoDock.Results:As revealed by network pharmacology，the potential active components of Biyuan Tongqiao Granules in the treatment of AR were quercetin，apigenin，kaempferol，ursolic acid，and rosmarinic acid，which regulated signaling pathways such as MAPK，HIF-1，NF-κB，and cAMP by mediating PTGS2，PTGS1，CHRM2，ADRB2，NOS2，and other targets，thereby exerting the anti-inflammatory effect.Molecular docking results showed that the key components had good affinity with PTGS1，PTGS2，and IgE antibodies.Conclusion:Based on network pharmacology and molecular docking technology，this study revealed that Biyuan Tongqiao Granules regulated AR through multiple components，multiple targets，and multiple systems，and provide a basis for clinical drug treatment. |
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