| 引用本文:王振亦1，常泽1，胡文悦1，林景峰1，王育纯1，马华萍1，刘甘露1，韩振蕴2.基于网络药理学对紫苏-百合药对治疗原发性失眠的作用机制研究[J].世界中医药,2022,(12):. |
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| 基于网络药理学对紫苏-百合药对治疗原发性失眠的作用机制研究 |
| Mechanism of Folium Perillae-Bulbus Lilii Combination against Primary Insomnia:Based on Network Pharmacology |
| 投稿时间：2021-01-09 |
| DOI：10.3969/j.issn.1673-7202.2022.12.004 |
| 中文关键词: 紫苏 百合 网络药理学 原发性失眠 不寐 基因靶点 信号通路 化合物成分 |
| English Keywords:Folium Perillae Bulbus Lilii Network pharmacology Primary insomnia Insomnia Gene targets Signaling pathways Chemical components |
| 基金项目:国家重点研发计划项目(2019YFC1710103)——阈下抑郁、失眠、轻度认知障碍中医特色干预技术的临床评价研究 |
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| 中文摘要: |
| 目的：通过网络药理学研究紫苏-百合药对治疗原发性失眠的有效活性成分及相应基因靶点、信号通路，并探讨其作用机制。方法：通过中药系统药理学数据库与分析平台（TCMSP）、中药综合数据库（TCMID）和文献检索紫苏-百合药对的主要化合物及其相应的作用靶点；通过GeneCards、人类孟德尔遗传数据库(OMIM)检索原发性失眠的疾病靶点；紫苏-百合药对和原发性失眠的交集基因靶点通过String数据库和Cytoscape软件构建网络可视化；利用DAVID数据库对交集靶点进行基因本体（GO）富集分析和京都基因和基因组百科全书（KEGG）富集分析。结果：本研究共筛选到紫苏-百合药对主要有效活性成分共22个，其中紫苏16个，百合7个（包含1个重复活性成分）；有效活性成分与原发性失眠共同作用靶点53个；GO富集分析结果显示生物过程（BP)共有284条；细胞组分(CC)共有35条；分子功能(MF)共有58条；KEGG信号通路富集结果显示102条通路。结论：紫苏-百合药对能够通过多靶点、多通路治疗原发性失眠，为深入揭示其作用机制提供了生物信息学基础。 |
| English Summary: |
| To investigate the active components，corresponding gene targets and signaling pathways of Folium Perillae-Bulbus Lilii combination in the treatment of primary insomnia by network pharmacology，and to explore the mechanism.Methods:Main components and targets of the medicinal pair were searched from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)，Traditional Chinese Medicine Integrated Database(TCMID) and literature，and targets related to primary insomnia from GeneCards and Online Mendelian Inheritance in Man(OMIM).The common targets of the pair and the disease were visualized by String and Cytoscape.DAVID was employed for Gene Ontology(GO) term enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment of the common targets.Results:A total of 22 major active components of the pair were screened out，with 16 from Folium Perillae and 7 from Bulbus Lilii(1 shared by the two medicinals)，and 53 common targets of the pair and the disease were identified.The common targets were involved in the GO terms of 284 biological processes(BPs)，35 cellular components(CCs)，and 58 molecular functions(MFs)，and 102 KEGG pathways.Conclusion:The combination of Folium Perillae and Bulbus Lilii exerts therapeutic effect on primary insomnia through multiple targets and multiple pathways，which lays a bioinformatic basis for further revealing its mechanism of action. |
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