世界中医药

目的：探讨补骨脂素基于Wnt/β-连环素（β-Catenin）信号通路对骨质疏松症（OS）大鼠的影响。方法：将78只SD大鼠随机分为对照组13只及造模组65只。造模组用双卵巢切除法建立OS模型，对照组予以假手术。造模成功者分为模型组、阿仑膦酸钠组及补骨脂素低、中、高浓度组，每组13只。模型组及对照组给予双蒸水，阿仑膦酸钠组给予0.2 mg/mL阿仑膦酸钠，补骨脂素低、中、高浓度组分别予0.4 mg/mL、0.8 mg/mL、1.6 mg/mL补骨脂素；各组灌胃剂量5 mL/kg，连续12周。检测比较骨密度（BMD），骨小梁厚度（Tb.Th）、数量（Tb.N）及分离度（Tb.Sp），血清及骨组织护骨因子（OPG）、骨钙蛋白（BGP）水平，以及骨组织低密度脂蛋白受体相关蛋白6（Lrp6）、糖原合酶激酶3β（GSK-3β）、β-catenin、Runt相关转录因子2（Runx2）mRNA表达水平。结果：与模型组比较，各组第4腰椎及左、右股骨BMD较高，Tb.Th、Tb.N较高、Tb.Sp较低，血清及骨组织OPG水平较高，BGP较低，骨组织Lrp6、β-catenin、Runx2 mRNA水平较高、GSK-3β mRNA较低，且补骨脂素组呈一定量效关系，差异有统计学意义（P<0.05）。结论：补骨脂素能剂量依赖性地提高OS大鼠BMD，提高血清及骨组织OPG水平，降低BGP水平，增加骨组织骨小梁厚度和数量，降低骨小梁分离度，其作用可能与激活Wnt/β-catenin信号通路有关。

English Summary:

To analyze the effect of psoralen on osteoporosis(OS) rats based on Wnt/β-catenin signaling pathway.Methods:A total of 78 SD rats were randomized into the control group(n=13) and modeling group(n=65).Bilateral ovariectomy was performed to induce OS in rats in the modeling group and rats in the control group received sham operation.The model rats were randomly classified into model group，alendronate sodium group，and low-concentration，medium-concentration，and high-concentration psoralen groups，with 13 rats in each group.The model group and control group were given double distilled water and alendronate sodium group received 0.2 mg/mL alendronate sodium.The low-concentration，medium-concentration，and high-concentration psoralen groups were treated with 0.4 mg/mL，0.8 mg/mL，and 1.6 mg/mL psoralen，respectively.Each group received intragastric administration at 5 mL/kg，qd，for 12 weeks.The bone mineral density(BMD)，trabecular thickness(Tb.Th)，trabecular number(Tb.N)，trabecular seaparation(Tb.Sp)，levels of osteoprotegerin(OPG) and osteocalcin(BGP) in serum and bone tissue，and expression of low density lipoprotein receptor-related protein 6(Lrp6)，glycogen synthase kinase 3 beta(GSK-3β)，β-catenin，and runt-related transcription factor 2(Runx2) in bone tissue were detected and compared.Results:Compared with model group，the administration groups showed high BMD of the 4th lumbar spine and left and right femur，large Tb.Th and Tb.N，low Tb.Sp，high OPG level and low BGP level in serum and bone tissue，high mRNA level of Lrp6，β-catenin，and Runx2，and low GSK-3β mRNA level，and the changes of the above indexes were dose-dependent in psoralen groups，with significant difference(P<0.05).Conclusion:Psoralen can increase BMD and level of OPG in serum and bone tissue，decrease the level of BGP，raise Tb.Th and Tb.N，and reduce Tb.Sp in OS rats in a dose-dependent manner.The mechanism is the likelihood that it activates the Wnt/β-catenin signaling pathway.

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