| 引用本文:高炜，徐艳霞，刘圆圆，徐媺.小檗碱调控核因子κB信号转导通路对幽门螺杆菌感染人胃癌细胞的影响[J].世界中医药,2022,(12):. |
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| 小檗碱调控核因子κB信号转导通路对幽门螺杆菌感染人胃癌细胞的影响 |
| Effect of Berberine Regulation of NF-κB Signaling Pathway on Hp-Infected Human Gastric Cancer Cells |
| 投稿时间：2020-08-14 |
| DOI：10.3969/j.issn.1673-7202.2022.12.011 |
| 中文关键词: 胃癌 幽门螺杆菌 细胞增殖 炎症反应 核因子κB信号转导通路 小檗碱 白细胞介素-4 白细胞介素-6 |
| English Keywords:Gastric cancer Helicobacter pylori Cell proliferation Inflammatory response NF-κB signaling pathway Berberine Interleukin-4 Interleukin-6 |
| 基金项目:山东省中医药科技发展计划项目(2017-341) |
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| 中文摘要: |
| 目的：研究小檗碱调控核因子κB信号转导通路对幽门螺杆菌（Hp）感染人胃癌细胞的影响。方法：建立Hp感染人胃癌细胞模型，并将其分成对照组、黄连素组、5-氟尿嘧啶组以及黄连素+5-氟尿嘧啶组。其中，对照组则予以等量生理盐水干预；黄连素组予以100 μg/mL浓度小檗碱干预；5-氟尿嘧啶组予以5 μg/mL的5-氟尿嘧啶干预；黄连素+5-氟尿嘧啶组则同时予以上述剂量的小檗碱以及5-氟尿嘧啶干预。比较各组Hp感染人胃癌细胞增殖情况，细胞周期的改变，核因子κB信号通路相关蛋白表达，炎症反应指标水平。结果：对照组、黄连素组、5-氟尿嘧啶组以及黄连素+5-氟尿嘧啶组干预后2 d、3 d时的胃癌细胞增殖率呈逐渐下降趋势；对照组、黄连素组、5-氟尿嘧啶组以及黄连素+5-氟尿嘧啶组感染人胃癌细胞G0/G1期、S期呈逐渐下降趋势，而G2/M期呈逐渐升高趋势；对照组、黄连素组、5-氟尿嘧啶组以及黄连素+5-氟尿嘧啶组IκB激酶α蛋白表达水平呈逐渐升高趋势，而P65蛋白表达水平呈逐渐降低趋势；对照组、黄连素组、5-氟尿嘧啶组以及黄连素+5-氟尿嘧啶组IL-4水平呈逐渐升高趋势，而白细胞介素-6呈逐渐下降趋势，各组比较差异均有统计学意义（均P<0.05）。结论：小檗碱主要是通过调控核因子κB信号转导通路相关蛋白的表达，继而发挥抑制Hp感染人胃癌细胞增殖与炎症反应的作用,值得临床重点关注。 |
| English Summary: |
| To investigate the effect of berberine regulation of the nuclear factor κB(NF-κB) signaling pathway on human gastric cancer cells infected with Helicobacter pylori(Hp).Methods:The Hp-infected human gastric cancer cell model was established and the cells were divided into a control group(normal saline)，a berberine group(100 μg/mL)，a 5-fluorouracil(5-Fu) group(5 μg/mL)，and a combination group(100 μg/mL berberine+5 μg/mL 5-Fu).The proliferation，cell cycles，protein expression related to the NF-κB signaling pathway，and inflammatory response index levels of Hp-infected human gastric cancer cells in the four groups were compared.Results:The proliferation rates of gastric cancer cells in all groups showed a decreasing trend on the 2nd day and 3rd day of the intervention.The Hp-infected human gastric cancer cells in the G0/G1 and S phases in all groups showed a gradually decreasing trend，while those in the G2/M phase showed a gradually increasing trend.The expression level of IκBα protein in all groups showed a gradually increasing trend，while the expression level of P65 protein showed a gradually decreasing trend.The level of IL-4 in all groups showed a gradually increasing trend，while the level of IL-6 showed a gradually decreasing trend.There were significant differences among all groups(all P<0.05).Conclusion:Berberine inhibits the proliferation and inflammatory response of Hp-infected human gastric cancer cells by regulating the expression of proteins related to the NF-κB signaling pathway，which is worthy of clinical attention. |
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