The biosynthesis pathway of flavonoids in Scutellaria baicalensis has been elucidated,but the regulation mechanism has not been thoroughly investigated.In this study,SbMYB transcription factors were systematically identified and analyzed based on the whole genome of S.baicalensis to screen candidate SbMYB members that may be involved in the biosynthesis of flavonoids.Methods:SbMYB members were screened out by HMMER and BLAST.IQ-Tree,ExPASy,GSDS,TBtools,Cytoscape,and other online tools and software were used to analyze the evolutionary relationship,gene structure,gene expression pattern,and regulatory network of SbMYB transcription factors.Results:A total of 146 SbMYB transcription factors were identified at the genome-wide level,which were divided into four subfamilies (one SbMYB was unclassified).Specifically,R2R3-MYB subfamily contained the most members (n=116).SbMYB genes were generally conservative in structure and randomly distributed on nine chromosomes.The same subfamily showed similar MYB conserved domains and motifs.The expression patterns of SbMYB genes were similar in roots,stems,and leaves,but significantly different from that in flowers.Forty-three SbMYB members that might regulate the flavonoid biosynthesis pathway were screened out by the protein-protein interaction analysis of SbMYB and structural genes related to flavonoid biosynthesis.Conclusion:MYB transcription factors regulate secondary metabolic synthesis in plants.This study systematically analyzed the MYB transcription factor family of S.baicalensis and screened candidate genes that might be involved in flavonoid biosynthesis to lay the foundation for the exploration of its regulation mechanism.