| 引用本文:王晓童1,2，向丽1，邬兰1，高冉冉1.黄芩MYB转录因子家族全基因组鉴定与分析[J].世界中医药,2022,(13):. |
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| 黄芩MYB转录因子家族全基因组鉴定与分析 |
| Genome-wide Identification and Analysis of MYB Transcription Factor Gene Family in Scutellaria baicalensis |
| 投稿时间：2022-05-10 |
| DOI：10.3969/j.issn.1673-7202.2022.13.003 |
| 中文关键词: 黄芩 MYB转录因子 黄酮类化合物 基因调控网络 |
| English Keywords:Scutellaria baicalensis MYB transcription factor Flavonoids Gene regulatory networks |
| 基金项目:国家自然科学基金委-贵州喀斯特中心项目（U1812403-1）；国家重点研发计划项目（2019YFC1711100） |
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| 中文摘要: |
| 目的:黄芩中主要活性成分黄酮类化合物的生物合成途径已解析，但相关调控机制研究薄弱。本研究基于黄芩全基因组对SbMYB转录因子进行系统鉴定和分析，筛选可能参与黄酮类化合物生物合成的候选SbMYB家族成员。方法:通过HMMER和BLAST筛选SbMYB转录因子；采用IQ-TREE、ExPASy、GSDS、TBtools、Cytoscape等在线工具和软件对SbMYB家族的进化关系、基因结构、基因表达模式、蛋白调控网络等进行生物信息学分析。结果：全基因组水平上共鉴定到146个SbMYB转录因子，分为4个亚族和1个未分族成员，R2R3-MYB亚族成员最多（116个）；SbMYB基因结构较为保守，随机分布在9条染色体上；同一亚族有相似的MYB保守结构域和基序组成；SbMYB在根、茎、叶中具有相似的表达模式，与花中差异较大；SbMYB与黄酮合成相关结构基因的蛋白质-蛋白质相互作用分析筛选到43个SbMYB可能调控黄酮合成途径关键酶。结论:MYB转录因子调控植物次生代谢合成，本研究系统分析黄芩MYB转录因子家族并筛选可能参与黄酮类化合物生物合成的候选基因，为其调控机制研究奠定基础。 |
| English Summary: |
| The biosynthesis pathway of flavonoids in Scutellaria baicalensis has been elucidated,but the regulation mechanism has not been thoroughly investigated.In this study,SbMYB transcription factors were systematically identified and analyzed based on the whole genome of S.baicalensis to screen candidate SbMYB members that may be involved in the biosynthesis of flavonoids.Methods:SbMYB members were screened out by HMMER and BLAST.IQ-Tree,ExPASy,GSDS,TBtools,Cytoscape,and other online tools and software were used to analyze the evolutionary relationship,gene structure,gene expression pattern,and regulatory network of SbMYB transcription factors.Results:A total of 146 SbMYB transcription factors were identified at the genome-wide level,which were divided into four subfamilies (one SbMYB was unclassified).Specifically,R2R3-MYB subfamily contained the most members (n=116).SbMYB genes were generally conservative in structure and randomly distributed on nine chromosomes.The same subfamily showed similar MYB conserved domains and motifs.The expression patterns of SbMYB genes were similar in roots,stems,and leaves,but significantly different from that in flowers.Forty-three SbMYB members that might regulate the flavonoid biosynthesis pathway were screened out by the protein-protein interaction analysis of SbMYB and structural genes related to flavonoid biosynthesis.Conclusion:MYB transcription factors regulate secondary metabolic synthesis in plants.This study systematically analyzed the MYB transcription factor family of S.baicalensis and screened candidate genes that might be involved in flavonoid biosynthesis to lay the foundation for the exploration of its regulation mechanism. |
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