To establish multi-index comprehensive quality evaluation and fingerprint method for the evaluation of the quality of 15 batches of Saposhnikovia divaricata samples from different origins.Methods:The prim-O-glucosylcimifugin,5-O-methylvisammioside,extract,and ash content in S.divaricata were determined by using the content determination method,alcohol-soluble extract method,total ash method,and acid-insoluble ash method.The data were analyzed,and a comprehensive quality evaluation function was established.Meanwhile,the fingerprints of S.divaricata from different origins were established by high-performance liquid chromatography (HPLC),and the data were analyzed by the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine and statistical software.Results:The RSD of extract,content,and ash content of S.divaricata from Henan,Hebei,Shanxi,and Inner Mongolia were in the range of 5.01%～14.90%.The results of comprehensive quality evaluation function showed that the quality of S.divaricata from Henan was optimal.The similarity of fingerprints of S.divaricata from different origins was 0.926～0.990,and the results of principal component analysis of common peak area showed that the quality of S.divaricata from Henan was optimal.Conclusion:There were differences in the quality of S.divaricata in different producing areas.The quality of S.divaricata produced in Henan was optimal,and Henan can serve as a high-quality producing area of S.divaricata.