| 引用本文:曹玲华1,2，肖光旭1,2，贺爽1,2，朱彦1,2.防己黄芪汤抗慢性心力衰竭的作用及靶标分析[J].世界中医药,2022,(13):. |
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| 防己黄芪汤抗慢性心力衰竭的作用及靶标分析 |
| Efficacy and Targets of Fangji Huangqi Decoction against Chronic Heart Failure |
| 投稿时间：2021-03-11 |
| DOI：10.3969/j.issn.1673-7202.2022.13.010 |
| 中文关键词: 网络药理学 防己黄芪汤 慢性心力衰竭 心肌肥大 信号通路 复方中药 活性成分 |
| English Keywords:Network pharmacology Fangji Huangqi Decoction Chronic heart failure Cardiac hypertrophy Signaling pathway Compound Chinese medicine Active components |
| 基金项目:国家重点研发计划项目(2018YFC1704502);国家自然科学基金项目(81873037) |
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| 中文摘要: |
| 目的：探讨中医药经典名方防己黄芪汤防治慢性心力衰竭的可能药效和作用靶点。方法：采用免疫荧光和RT-qPCR方法评价给药后H9c2细胞对去氧肾上腺素诱导的心肌肥大和慢性心力衰竭标志物心房利尿钠肽（ANP）和脑利尿钠肽（BNP）表达的影响。通过中药系统药理学数据库与分析平台（TCMSP）及文献调研收集防己黄芪汤的活性成分和作用靶点；从GeneCards等数据库获取慢性心力衰竭主要靶点，将疾病靶点和药物靶点取交集，利用STRING平台对潜在作用靶点进行相互作用分析，构建网络，采用DAVID以及ClusterProfiler包进行基因本体（GO）和京都基因和基因组百科全书（KEGG）通路分析，并使用Cytoscape 3.7.2软件构建网络图。结果：防己黄芪汤可缓解去氧肾上腺素诱导的心肌细胞增大，减低ANP和BNP基因的表达。防己黄芪汤成分和慢性心力衰竭交集的166个潜在作用靶点主要富集于493个生物过程和171个信号通路上。结论：防己黄芪汤具有防治慢性心力衰竭的体外药效，主要通过槲皮素、芒柄花黄素、粉防己碱、黄芪甲苷等活性成分作用于缺氧、炎症和代谢相关信号通路，达到防治慢性心力衰竭的目的。 |
| English Summary: |
| To explore the efficacy and potential targets of Fangji Huangqi Decoction against chronic heart failure.Methods:Immunofluorescence and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) were used to determine the expression of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP),the markers of phenylephrine-induced myocardial hypertrophy and chronic heart failure,in H9c2 cells.The active components and targets of Fangji Huangqi Decoction were retrieved from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) and previous research,and the main targets of chronic heart failure from Genecards and other databases.Thereby,the common targets of the disease and the decoction were screened out.The interaction between the targets was analyzed based on STRING and the protein-protein interaction (PPI) network was constructed.DAVID and ClusterProfiler were employed for Gene Ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment.Cytoscape 3.7.2 software was used to plot the networks.Results:Fangji Huangqi Decoction could alleviate the phenylephrine-induced myocardial hypertrophy and decrease the expression of ANP and BNP genes.The 166 anti-chronic heart failure targets of Fangji Huangqi Decoction were involved in 493 biological processes and 171 signaling pathways.Conclusion:Fangji Huangqi Decoction has therapeutic effect on chronic heart failure in vitro,mainly.To be specific,the active components quercetin,formononetin,tetrandrine,and astragaloside IV acts on hypoxia-,inflammation-,and metabolism-related signaling pathways,to prevent and treat chronic heart failure. |
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