| To explore the medication rule for lumbar disc herniation (LDH) and predict the mechanism based on network pharmacology.Methods:Related articles were searched from China National Knowledge Infrastructure (CNKI),followed by the analysis of properties,flavors,and meridian tropisms of related medicinals,association rules analysis,and clustering analysis based on Traditional Chinese Medicine Inheritance Computing System (TCMICS) to screen the core medicinals and prescriptions.The active components and potential targets of core medicinals were retrieved from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP),and targets of LDH from GeneCards and Online Mendelian Inheritance in Man (OMIM).STRING was employed to analyze the key targets of core medicinals against LDH,followed by Gene Ontology (GO) term enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment.Results:A total of 104 prescriptions were retrieved,involving 233 medicinals,and 12 core medicinals and 5 core prescriptions were screened out.The core medicinals were from the Bushen Huoxue Decoction,Shentong Zhuyu Decoction,and Liuwei Dihuang Decoction.A total of 178 effective active components,including quercetin,luteolin,and kaempferol,and 52 anti-LDH targets were obtained.According to the protein-protein interaction (PPI) network,the core targets were JUN,protein kinase B (AKT1),interleukin-6 (IL6),mitogen-activated protein kinase 1 (MAPK1),vascular endothelial growth factor A (VEGFA),FOS,mitogen-activated protein kinase 14 (MAPK14),etc.The characteristic target proteins of Bushen Huoxue Formulas,Ziyin Bushen Formulas,and Huoxue Huayu Formulas were dopamine receptor D3 (DRD3),insulin (INS),and vitamin D receptor (VDR),respectively.The Jianpi Bushen Formulas were closely related to interleukin 17A (IL17A) and tumor necrosis factor (TNF).The core target proteins of the Huoxue Tongluo Formulas were C-X-C motif chemokine ligand 8 (CXCL8) and nuclear factor kappa B subunit 1 (NFKB1).The key targets were involved in the following KEGG pathways:inflammation-related pathways of TNF,IL-17,MAPK,PI3K-Akt,and hypoxia-inducible factor-1 (HIF-1) pathways,and immune-related pathways such as Th17 cell differentiation and T cell receptor pathways.Conclusion:The treatment of LDH by kidney-tonifying and blood-activating method is often coordinated with dredging collaterals,invigorating spleen,and tonifying yin.The mechanism is the likelihood that it inhibits local inflammatory response and regulates systemic immune balance with multiple targets and multiple pathwways. |
