To screen out the main active components of Paridis Rhizoma[dried rhizome of Paris polyphylla var.yunnanensis(Franch.) Hand.-Mazz.] based on network pharmacology,predict the targets and signaling pathways in the treatment of non-small cell lung cancer(NSCLC),so as to decipher the possible mechanism of Paridis Rhizoma in treating NSCLC.Methods:From the Database of Chinese Medicine and Chemical Constituents,Swiss Institute of Bioinformatics(SIB),and relevant literature,the active components and corresponding targets of Paridis Rhizoma were extracted.GeneCards and Online Mendelian Inheritance in Man(OMIM) were used to obtain potential target genes of NSCLC.The common targets shared by Paridis Rhizoma and NSCLC were intersected as the key targets.The gene ontology(GO) annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis of the key targets were carried out based on DAVID,and the predicted results were verified by molecular docking.Results:Seven active components of Paridis Rhizoma were screened out,including β-ecdysone,γ-aminobutyric acid,polyphyllin A,polyphyllin G,polyphyllin F,polyphyllin D,and polyphyllin B.Seven key targets were screened out,including MAPK1,MAPK3,VEGFA,PIK3CA,BCL2L1,FGF1,and STAT3.Conclusion:The seven key targets play an important role in cancer treatment,immune regulation,and telomere stability through cancer pathway,NSCLC pathway,PI3K-AKT signaling pathway,FoxO signaling pathway,and MAPK signaling pathway.Based on the method of network pharmacology,this study predicted the possible mechanism of Paridis Rhizoma in the treatment of NSCLC and provided reference for further research.