To explore the optimal process of extracting lupeol from the seeds of Indigofera pseudotinctoria and the correlation between liquid chromatography-mass spectrometry(LC-MS) and gas chromatography-mass spectrometer(GC-MS) peak area.Methods:1) The L9(34) orthogonal experiment was used to optimize the optimal process of extracting lupeol from I.pseudotinctoria,and the peak area was detected by GC-MS.2) The content of the single factor experiment was detected by LC-MS,and the three solvents(95% ethanol,ethyl acetate,and petroleum ether) were extracted at 10 times liquid-material ratio for 5 and 15 days,respectively.The ratio of peak area was compared with that of the orthogonal experiment,and the content of lupeol in the orthogonal experiment was deduced.Results:1) After GC-MS detection,the content and median of the samples obtained from 26 processing routes in the orthogonal experiment were tested,and the median value was 4 503.655 μg.The petroleum ether extracted for 5 days at 10 times liquid-material ratio obtained the highest content of lupeol,and the 95% ethanol extracted for 5 days at 5 times liquid-material ratio obtained the lowest content of lupeol.The 95% ethanol extracted for 5 days at 10 times liquid-to-material ratio obtained the highest content of lupeol after excluding petroleum ether.2) The GC-MS values of the samples under the same process were deduced by content,and the difference had no statistical significance(P>0.05).The corresponding ratios of 95% ethanol,ethyl acetate,and petroleum ether were 315.91,208.25,and 1 305.83,respectively.Conclusion:1) From the perspective of safety,economy,and industrial production,it is superior to use 5% ethanol to extract lupeol from the seeds of I.pseudotinctoria at 10 times liquid-material ratio for 15 days.2) GC-MS often qualifies the presence or absence of a certain component,but it is not used as a quantitative basis.Information about multiple components can be obtained at one time.LC-MS is generally used as a quantitative basis,but only one component can be quantified at one time.From this experiment,it seems that the peak area value of GC-MS can be deduced from LC-MS,which provides a convenient method for preliminary judging the peak area value of GC-MS,and it is worth further accumulating data and finding out the rules.