To study the model evaluation of nitroglycerin(NTG)-induced migraine-like pain behavior in rats and explore the pharmacodynamical effect of Piantongtang No.1(Pttn1) on the rat model of migraine.Methods:The model was induced by subcutaneous injection of NTG in the neck of rats.The rats with successful models were randomly divided into a model group,a positive drug group[0.25 mg/(kg·d)],a transient receptor potential cation channel subfamily V member 1(TRPV1) inhibitor group [3 mg/(kg·d)],a Pttn1 group [13.5 g/(kg·d)],a Pttn1+TRPV1R group,a sham operation group,and a control group,with 12 rats(half male and half female) in each group.Each group was treated with drugs for 1 week,and the behavior and pain threshold changes were detected at the same time.Blood was taken 1 h after the last administration,and the expression levels of histamine(HA) and 5-hydroxytryptamine(5-HT) were determined by enzyme-linked immunosorbent assay(ELSIA).Results:As compared with the control group,the scores of behaviors such as head scratching and to and fro movement in the model group increased,while the score of cage climbing behavior only increased significantly after the second modeling and the pain threshold decreased(P<0.05,P<0.01).As compared with the model group,the score of head scratching and plasma HA content in the Pttn1 group decreased after intragastric administration,the pain threshold and 5-HT content up-regulated,and the to and fro movement was reduced in the Pttn1 group only on the first day(P<0.05,P<0.01).As compared with the TRPV1 inhibitor group,the hyperalgesia of the Pttn1 group was not significant on the 6th day(P<0.05).Conclusion:This experiment successfully replicates the rat model of migraine,and the female rat model has significant changes.The Pttn1 obviously improves the migraine-like behavior,hyperalgesia,and plasma vasoactive amine changes in the rat model.