To observe the effects of Qichen Granules(QG) on the extracellular matrix(ECM) metabolism of cardiac fibroblasts(CFs) induced by Angiotensin Ⅱ(Ang Ⅱ)，and explore the molecular mechanism of QG against myocardial fibrosis(MF).Methods:The passage cultured primary CFs were divided into a blank control group，an Ang Ⅱ group，a captopril group，and a QG group.After intervention for 48 h，the secretions and mRNA expression levels of matrix metalloproteinase(MMP)-2，MMP-9，type I collagen(Col Ⅰ)，type Ⅲ collagen(Col Ⅲ)，tissue inhibitor of metalloproteinase(TIMP)-1，and TIMP-2 were determined.Results:The Ang Ⅱ group had a higher proliferation of CFs than the blank control group，and the captopril group and the QG group had a lower proliferation of CFs than the Ang Ⅱ group(P<001).The content and mRNA expression levels of Col Ⅰ and Col Ⅲ were increased in the Ang Ⅱ group as compared with the blank control group but decreased in the captopril group and the QG group as compared with the Ang Ⅱ group(P<001).The expressions of MMP-2，MMP-9，TIMP-1，and TIMP-2 and the ratios of MMP2/TIMP2 and MMP9/TIMP1 were increased in the Ang Ⅱ group as compared with the blank control group，decreased in the captopril group and the QG group as compared with the Ang Ⅱ group(P>005)，and were lower in the GQ group as compared with the captopril group(P<001).Conclusion:QG can inhibit the proliferation of Ang Ⅱ-induced CFs and reduce the expressions of Col Ⅰ and Col Ⅲ，which has a better effect than captopril.The mechanism may be related to the down-regulation of the expressions of MMP-2，MMP-9，TIMP-1，and TIMP-2，the reduction of the ratios of MMP-2/TIMP-2 and MMP-9/TIMP-1，and the regulation of matrix metabolism.