To explore the therapeutic mechanism of Astragali Radix on ischemic stroke(IS) by constructing network relationship between the drug and the disease.Methods:The active components and related targets of Astragali Radix were retrieved in the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).The gene targets of IS were retrieved from GeneCards and Online Mendelian Inheritance in Man(OMIM) with the term “cerebral ischemic stroke”.Perl script was used to analyze and calculate the common genes of Astragali Radix and IS.String was used to construct the protein-protein interaction(PPI) network of Astragali Radix and IS.The GO function enrichment analysis and KEGG pathway enrichment analysis of common genes of Astragali Radix and IS were carried out by R software.Results:Sixteen active components of Astragali Radix were screened out,which acted on 137 IS-related targets.The core genes of Astragali Radix in the treatment of IS mainly included AKT1,IL6,VEGFA,CASP3,and JUN.The targets of the drug and the disease involved 144 GO biological processes,and their functions were mainly related to cell activity,specific DNA expression,and enzyme reactions.A total of 164 KEGG signaling pathways were obtained,including AGE-RAGE signaling pathway,hepatitis B virus pathway,prostate cancer pathway,TNF signaling pathway,human cytomegalovirus infection pathway,bladder cancer pathway,pancreatic cancer pathway,hepatitis C pathway,and endocrine resistance pathway.Conclusion:Astragali Radix can treat IS through multiple components,multiple targets,and multiple pathways,which provides certain scientific theoretical support for Astragali Radix in the treatment of IS.