| To analyze the mechanism of Testudinis Carapacis et Plastri Colla(TCPC) in the treatment of perimenopausal insomnia based on network pharmacology.Methods:The chemical components and target genes of TCPC were retrieved from Bioinformatics Analysis Tool for Molecular Mechanism of Traditional Chinese Medicine(BATMAN-TCM),and target genes related to the treatment of perimenopausal insomnia were obtained from GeneCards and Online Mendelian Inheritance in Man(OMIM).The common target genes of the disease and the drug were obtained by virtue of Venny2.1.The disease-target gene-pathway-component network was plotted using Cytoscape 3.7.2.The protein-protein interaction(PPI) network of target genes was constructed using String,and the target genes underwent GO function and KEGG pathway enrichment analyses by R software.Results:According to the database retrieval and analysis of network pharmacology,six active components in TCPC interacted with 21 key target genes related to perimenopausal insomnia,including ASPA,MPO,PPIG,EDN1,and GLRA1.GO functional enrichment analysis yielded a total of 592 items,including 514 biological processes,49 cellular components,and 29 molecular functions,mainly involving catecholamine metabolism,neurotransmitter level regulation,synaptic transmission,dopamine,amino acid binding,and neurotransmitter receptor activity.KEGG pathway enrichment analysis screened out 11 signaling pathways,mainly including dopaminergic synapses,amphetamine addiction,cAMP signaling pathway,and TNF signaling pathway.Conclusion:TCPC can treat perimenopausal insomnia through multiple targets and multiple pathways,which provides a reference for further exploration of its mechanism of action. |
