To explore the regularity of acupoint selection in ancient literature for acupuncture and moxibustion treatment of amenorrhea.Methods:The prescription information of acupuncture and moxibustion treatment of amenorrhea was retrieved from the database of the 5th edition of Chinese Medical Canon with 30 key terms such as “不月(Buyue,menstrual disorder)”,“月事不来(Yueshi Bulai,absence of menses”,“闭经(Bijing,amenorrhea)”,and “血枯(Xueku,blood exhaustion)”,screened out,and extracted.Frequency statistics,community analysis,association analysis,and complex network analysis of acupoint selection protocols for acupuncture and moxibustion treatment of amenorrhea were carried out by bibliometrics based on the Ancient and Modern Medical Case Cloud Platform(V2.3).Results:A total of 202 acupoint prescriptions meeting the requirements were selected,including 27 compound prescriptions and the rest were single-point prescriptions involving 38 acupoints with the frequency of 268.In terms of frequency,the top 15 acupoints for the treatment of amenorrhea were Zhongji,Xuehai,Shuiquan,Yinjiao,Qihai,Guanyuan,Zhaohai,Daimai,Yaoshu,Zhongliao,Qichong,Taichong,Sanyinjiao,Hegu,and Huiyin.As revealed by the complex network analysis,the core prescriptions were Zusanli,Sanyinjiao,Shenshu,Zhongji,Xuehai,Hegu,Zhaohai,Qichong,Qihai,and Yinjiao.Conclusion:The acupuncture and moxibustion treatment of amenorrhea has regularity in the selection of acupoints.The results of this study can provide a reference for clinical practice,and it is feasible to explore the ancient acupuncture and moxibustion literature through data mining technology.