| 引用本文:赵苑伶1,2，张雪1,2，陈林珍1,2，刘妍1,2，刘静1,2，赵崇军1,2，刘永刚1，马志强1,2.铁皮石斛多糖的分离纯化及抗衰老活性研究[J].世界中医药,2022,(17):. |
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| 铁皮石斛多糖的分离纯化及抗衰老活性研究 |
| Separation，Purification，and Anti-aging Activity of Caulis Dendrobii Officinalis Polysaccharides |
| 投稿时间：2021-10-11 |
| DOI：10.3969/j.issn.1673-7202.2022.17.007 |
| 中文关键词: 铁皮石斛 多糖 分离纯化 秀丽隐杆线虫 寿命 抗氧化 抗衰老 |
| English Keywords:Caulis Dendrobii Officinalis Polysaccharides Separation and purification Caenorhabditis elegans Lifespan Antioxidation Anti-aging |
| 基金项目:国家重点研发计划项目（2018YFC1706500） |
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| 中文摘要: |
| 目的：分离纯化铁皮石斛多糖（DOP），得到纯化后的铁皮石斛多糖DOP-1，并以秀丽隐杆线虫为模式生物，研究DOP-1的抗衰老作用。方法：以铁皮石斛鲜品为原料，经提取、浓缩、乙醇沉淀和真空干燥后，得到铁皮石斛粗多糖，铁皮石斛粗多糖经DEAE-Sepharose Fast Flow层析柱和HW-55F凝胶柱洗脱后获得纯品铁皮石斛多糖DOP-1，通过高效凝胶渗透色谱法测定其纯度及相对分子质量等参数，同时研究不同浓度DOP-1（100 μg/mL、200 μg/mL、400 μg/mL）对秀丽隐杆线虫寿命、生殖能力、抗高温损伤、氧化应激损能力及脂褐素的影响。结果：分离纯化后的DOP-1为均一性多糖，其相对分子质量为3.260×105，不同浓度DOP-1均可以显著延长秀丽隐杆线虫的寿命（P<0.05或P<0.01），其中以200 μg/mL DOP-1作用最为明显，与对照组比较，寿命延长了43.37%，DOP-1对高温损伤和氧化应激损伤有一定的抵御作用，能有效降低秀丽隐杆线虫体内脂褐素的积累，并对其生殖能力无明显影响。结论：分离纯化后的DOP-1为均一性多糖，并具有较显著的抗衰老作用，可对其结构与抗衰老活性之间关系的研究提供参考。 |
| English Summary: |
| To separate and purify Caulis Dendrobii Officinalis polysaccharides(DOP) to obtain DOP-1 and to study the anti-aging effect of DOP-1 with Caenorhabditis elegans.Methods:Fresh Caulis Dendrobii Officinalis was extracted and the extract was concentrated，followed by ethanol precipitation and vacuum drying to yield crude DOP.Crude DOP was separated on DEAE-Sepharose Fast Flow column and eluted with HW-55F gel column to result in the pure DOP-1.Parameters such as purity and relative molecular weight were determined by high-performance gel-permeation chromatography.Moreover，the effect of different concentration of DOP-1(100 μg/mL，200 μg/mL，400 μg/mL) on lifespan，reproductive ability，and lipofuscin accumulation of C.elegans，and the resistance of C.elegans to high temperature and oxidative stress was investigated.Results:The isolated and purified DOP-1 was a homogeneous polysaccharide with relative molecular weight of 3.260 × 105 Da.Different concentration of DOP-1 could significantly prolong the lifespan of C.elegans(P<0.05 or P<0.01)，particularly the 200 μg/mL DOP-1 which increased the lifespan by 43.37% compared with the control group.DOP-1 can protect C.elegans from high temperature injury and oxidative stress injury and effectively reduce the accumulation of lipofuscins.However，it has no remarkable influence on the reproductive ability of the C.elegans.Conclusion:The isolated and purified DOP-1 is a homogenous polysaccharide with significant anti-aging effect，which can serve as a reference for the research on the relationship between its structure and anti-aging activity. |
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