| To observe the preventive and therapeutic effect and mechanism of Dingkun Pills on rats with intrauterine adhesion(IUA).Methods:Sixty SD rats were randomly divided into blank group,model group,estradiol valerate group,and Dingkun Pills low-,medium-and high-dose groups,with 10 in each group.IUA model was prepared by mechanical curettage and lipopolysaccharide(LPS) infection.Estradiol valerate group was given 0.3 mg/kg estradiol valerate by gavage,and Dingkun Pills low-,medium-and high-dose groups were given corresponding doses by gavage,once a day for 28 days.Hematoxylin and eosin(HE) staining and Masson staining were used to observe the morphological changes of rat uterine tissue and the area of endometrial fibrosis,respectively.Enzyme linked immunosorbent assay(ELISA) and immunohistochemistry were performed to detect the levels of serum vascular endothelial growth factor(VEGF),interleukin 6(IL-6) and interleukin 8(IL-8),and the expression of VEGF in endometrium,respectively.Western blot and reverse transcription-polymerase chain reaction(RT-PCR) were conducted to determine the protein and mRNA expression of phosphatidylinositol 3-kinase/protein kinase B/mammalian target of the rapamycin(PI3K/Akt/mTOR) pathway in uterine tissue,respectively.Results:Dingkun Pills could effectively promote the healing of injured uterus in IUA rats,reduce the area of uterine fibrosis,and decrease the levels of serum VEGF,IL-6 and IL-8,which were significantly different from the conditions in the model group(P<0.05).After treatment with various doses of Dingdan Pills,the expression of VEGF in rat endometrium was lower than that in the model group(P<0.05),and the protein and mRNA expression levels of PI3K,p-Akt and p-mTOR in uterine tissue were down-regulated(P<0.05).Conclusion:Dingkun Pills had a good therapeutic effect on IUA rats,and the mechanism might be related to down-regulating the protein expression of PI3K/Akt/mTOR signaling pathway,and inhibiting vascular proliferation and the secretion of inflammatory mediators. |
