| 引用本文:黄浩洲1，张定堃1，李梦琪2，谭鹏3，包晓明4，莫太刚5，樊三虎5，韩丽1，林俊芝6.基于顶空固相微萃取-气相色谱-三重四极杆质谱联用技术的余甘子挥发性成分分析及抗仓储害虫作用研究[J].世界中医药,2022,(19):. |
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| 基于顶空固相微萃取-气相色谱-三重四极杆质谱联用技术的余甘子挥发性成分分析及抗仓储害虫作用研究 |
| Volatile Components of Phyllanthus emblica L.and Its Effect Against Storage Pests Based on HS-SPME-GC-QQQ-MS/MS Odor Analysis Technology |
| 投稿时间：2022-08-26 |
| DOI：10.3969/j.issn.1673-7202.2022.19.001 |
| 中文关键词: 余甘子 精油 仓储害虫 抗虫 挥发性成分 超临界萃取 气味强度特征谱 |
| English Keywords:Phyllanthus emblica L Essential oil Storage pests Pest resistance Volatile components Supercritical extraction Odor intensity characteristic spectrum |
| 基金项目:国家自然科学基金项目(81973493)；四川省中医药标准化研究项目(2019073)；三勒浆药业-成中医联合实验室项目(2019-YF04-00086-JH) |
| 作者 | 单位 | | 黄浩洲1，张定堃1，李梦琪2，谭鹏3，包晓明4，莫太刚5，樊三虎5，韩丽1，林俊芝6 | 1 成都中医药大学药学院/创新产业研究院,西南特色中药资源国家重点实验室,成都,611137
2 四川护理职业学院药学系,成都,610100
3 四川省中医药科学院国家中医药管理局中药质量生物评价重点研究室,成都,610041
4 岛津企业管理(中国)有限公司,成都,610023
5 成都市三勒浆药业集团,成都,610000
6 成都中医药大学附属医院代谢性疾病中医药调控四川省重点实验室,成都,610072 |
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| 中文摘要: |
| 目的：对余甘子挥发性成分进行富集分析，并评价其抗虫作用。方法：采用CO2超临界流体萃取法制备余甘子精油，采用顶空固相微萃取-气相色谱-三重四极杆质谱联用(HS-SPMEGC-QQQ-MSMS）技术构建了余甘子气味成分谱（OCS）和气味强度特征谱（OICS），对比余甘子药材粉末与精油的挥发性物质组成差异。在封闭空间内研究余甘子药材及精油对药材甲、咖啡豆象、米象、印度谷蛾等仓储害虫死亡率、进食率和趋避作用的影响。结果：药材和精油成分基本一致，但药材中小分子酸类等成分含量更高。余甘子药材与精油均对进食率影响不大，但能提高昆虫死亡率，趋避作用明显，且余甘子精油驱虫效果更强。结论：余甘子药材和精油具有一定的驱虫作用，乙酸乙酯、芳樟醇、α-松油醇等可能是其抗虫主要物质基础。本文首次报道了余甘子的抗虫作用，为余甘子精油的综合开发提供参考。 |
| English Summary: |
| To enrich and analyze the volatile components of Phyllanthus emblica L.and evaluate its pest resistance.Methods:The essential oil of P.emblica L.was prepared by CO2 supercritical fluid extraction，and the odor component spectrum(OCS) and odor intensity characteristic spectrum(OICS) of P.emblica L.were constructed by headspace sampling-solid-phase microextraction-gas chromatography-triple quadrupole-mass spectrometry/mass spectrometry(HS-SPME-GC-QQQ-MS/MS) technology.The difference in volatile components between the powder of P.emblica L.and its essential oil was compared.The effects of P.emblica L.and its essential oil on the mortality，feeding rate，and evading of storage pests such as Stegobium paniceum，Araecerus fasciculatus，Sitophilus oryzae，and Plodia interpunctella were studied in an enclosed space.Results:The components of the crude drug and the essential oil were basically the same，but the content of small molecular acids and other components in the crude drug was higher.Both P.emblica L.and essential oil have little effect on the feeding rate，but they can increase the mortality of pests with obvious evasive effects.The effect of P.emblica L.essential oil on pest repellency is stronger.Conclusion:P.emblica L.and its essential oil have certain pest-repellent effects.Ethyl acetate，linalool，and α-terpineol may be the main material basis of its pest resistance.This paper reports the pest resistance of P.emblica L.for the first time and provides references for the comprehensive development of P.emblica L.essential oil. |
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