| 引用本文:黄慧珍1，王松松1，林凡1,2，李晓冉1，苏陈颖1，林晓晖1,2，张捷平1,2.基于网络药理学探讨石斛治疗2型糖尿病的作用机制[J].世界中医药,2022,(19):. |
|
| 基于网络药理学探讨石斛治疗2型糖尿病的作用机制 |
| Mechanism of Dendrobii Caulis in Treatment of Type 2 Diabetes Based on Network Pharmacology |
| 投稿时间：2020-09-17 |
| DOI：10.3969/j.issn.1673-7202.2022.19.006 |
| 中文关键词: 石斛 2型糖尿病 网络药理学 作用机制 活性成分 靶蛋白 富集分析 信号通路 |
| English Keywords:Dendrobii Caulis Type 2 diabetes mellitus Network pharmacology Mechanism Active components Target protein Enrichment analysis Signaling pathway |
| 基金项目:国家自然科学基金项目(82074191);福建省自然科学基金项目(2020J01720);国家级大学生创新训练计划项目(201910393004) |
|
| 摘要点击次数: 557 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的:通过网络药理学方法分析石斛(Herba Dendrobii)在治疗2型糖尿病(T2DM)中的作用及相关分子机制。方法:通过检索有机小分子生物活性数据库(Pubchem)等数据库获取石斛已知活性成分及靶蛋白;利用人类基因数据库(GeneCards)获取T2DM相关疾病基因;运用Cytoscape 3.8.0软件分别构建“石斛活性成分-靶蛋白”相互作用网络和“石斛靶蛋白-T2DM基因”蛋白质-蛋白质相互作用(PPI)网络分析网络拓扑结构;采用OmicShare网站进行石斛治疗T2DM靶点京都基因和基因组百科全书(KEGG)富集分析和基因本体(GO)富集分析。结果:筛选获得石斛15个活性成分及其对应的57个靶蛋白,其中主要活性成分为木犀草素、芹菜素等,关键蛋白为雌激素受体α(ESR1)、酪蛋白激酶2α(CSNK2A1)等。GO富集结果提示石斛涉及代谢过程、对内源性刺激反应等生物学过程;参与酶结合、蛋白结合、转录因子结合等分子功能的调节。KEGG通路富集结果暗示,石斛可能通过作用于叉头框O(FOXO)、晚期糖基化终末产物-晚期糖基化终末产物受体(AGEs-RAGE)、磷脂酰肌醇3激酶/蛋白激酶B(PI3K/AKT)等信号通路,调节细胞炎症反应、氧化应激和胰岛素抵抗等,治疗T2DM。结论:石斛治疗T2DM涉及多个靶点和信号通路,可能通过抗氧化应激、抑制炎症反应和胰岛素抵抗等过程进行干预。 |
| English Summary: |
| To analyze the effect of Dendrobii Caulis on the treatment of type 2 diabetes mellitus (T2DM) and its related molecular mechanism by network pharmacology.Methods:The known active components and target proteins of Dendrobii Caulis were obtained through the retrieval of PubChem.GeneCards,the human gene database,was used to obtain T2DM-related disease genes.Cytoscape 3.8.0 was used to construct the “Dendrobii Caulis active component-target protein” interaction network and the “Dendrobii Caulis target-T2DM disease gene” protein-protein interaction (PPI) network,and the topology structure of the network was analyzed.OmicShare website was used to analyze the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment pathways of Dendrobii Caulis against T2DM.Results:Fifteen active components and 57 target proteins corresponding to Dendrobii Caulis were screened out,and the main active components were luteolin and apigenin.The key proteins were estrogen receptor 1 (ESR1) and casein kinase Ⅱ alpha (CSNK2A1).The GO enrichment results suggested that Dendrobii Caulis was involved in biological processes such as the metabolic process and the response to endogenous stimuli.It was involved in the regulation of molecular functions such as enzyme binding,protein binding,and transcription factor binding.The KEGG pathway enrichment results suggested that Dendrobii Caulis treated T2DM by acting on forkhead box O (FoxO),advanced glycation end-products-receptor advanced glycation end products (AGEs-RAGE),phosphoinositide 3-kinase/protein kinase B (PI3K/AKT),and other signaling pathways to regulate the cellular inflammatory response,oxidative stress,and insulin resistance.Conclusion:The treatment of T2DM with Dendrobii Caulis involves multiple targets and signaling pathways,which may be interfered with antioxidant stress,inhibition of inflammatory response,and insulin resistance. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
