To systematically study the medicinal substances and in vivo metabolism spectrum of Compound Ganmaoling Granules by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)，and to explore its mechanism in combination with network pharmacology.Methods:UPLC-MS/MS method was used to identify the main chemical components of Compound Ganmaoling Granules and the components absorbed in blood.Network pharmacology was used to construct the “component-target-efficacy-disease” network，and core targets were screened via protein-protein interaction(PPI) network for gene ontology(GO) and Kyoto Encyclopedia of Gene and Genome(KEGG) pathway enrichment analysis.Results:A total of 170 compounds and 21 components absorbed in blood were identified from the ethanol extract of Compound Ganmaoling Granules to construct the component spectrum and metabolism spectrum.The predicted core targets were tumor necrosis factor(TNF)，vascular endothelial growth factor A(VEGFA)，epidermal growth factor receptor(EGFR)，Caspase 3(CASP3)，prostaglandin-peroxidase synthase 2(PTGS2)，mitogen-activated protein kinase 1(MAPK1)，signal transducer and activator of transcription 3(STAT3)，matrix metalloproteinase 9(MMP 9)，interleukin-2(IL-2) and mitogen-activated protein kinase 14(MAPK14)，corresponding to antipyretic，anti-inflammatory and antiviral effects.Conclusion:This study constructed the component spectrum and in vivo metabolism spectrum of Compound Ganmaoling Granules using UPLC-MS/MS，and clarified its overall regulation by multiple components and targets from the perspective of network pharmacology，which preliminary reveals the antipyretic，anti-inflammatory and antiviral basis and mechanism，laying a foundation for the in-depth study of Compound Ganmaoling.