| 引用本文:何梦如1，王昊2，刘雅洁1，黄跃平1，吴嘉威1，赵百孝3.艾烟和香烟对APP/PS1双转基因小鼠海马β-淀粉样蛋白及血清炎症介质的影响[J].世界中医药,2022,(22):. |
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| 艾烟和香烟对APP/PS1双转基因小鼠海马β-淀粉样蛋白及血清炎症介质的影响 |
| Effect of Moxa Smoke and Cigarette Smoke on Hippocampal β-amyloid and Serum Inflammatory Factors in APP/PS1 Mice |
| 投稿时间：2021-11-05 |
| DOI：10.3969/j.issn.1673-7202.2022.22.003 |
| 中文关键词: 阿尔茨海默病 艾烟 香烟 神经炎症 β-淀粉样蛋白 炎症介质 APP/PS1双转基因小鼠 |
| English Keywords:Alzheimer's disease Moxa smoke Cigarette smoke Neuroinflammation β-amyloid Inflammatory factors APP/PS1 transgenic mice |
| 基金项目:国家自然科学基金面上项目（82174507，81574068）；北京中医药大学基本科研业务费项目（2020-JYB-XJSJJ-019） |
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| 中文摘要: |
| 目的：观察艾烟和香烟对APP/PS1双转基因小鼠海马β-淀粉样蛋白及血清炎症介质的影响，从神经炎症角度探讨艾烟防治阿尔茨海默病(AD)的作用机制。方法：将36只24周龄雄性APP/PS1小鼠按随机数字表法分为模型组、艾烟组、香烟组，12只相同遗传背景的C57BL/6小鼠作为空白组。艾烟组和香烟组分别采用5~15 mg/m3浓度的艾烟及香烟进行干预，空白组和模型组仅抓取固定。各组小鼠干预时间为20 min/d，6 d/周，连续干预8周。采用刚果红染色法观察各组小鼠海马β-淀粉样蛋白沉积情况，采用酶联免疫吸附试验法（ELISA）检测血清中肿瘤坏死因子-α（TNF-α）、白细胞介素-8（IL-8）、血清淀粉样蛋白A（SAA）、C反应蛋白（CRP）含量。结果：刚果红染色结果显示，与空白组比较，模型组和香烟组见大量β-淀粉样蛋白斑块沉积，艾烟组偶见散在β-淀粉样蛋白沉积颗粒。与空白组比较，模型组血清TNF-α、IL-8、CRP含量均明显升高（P<0.05），香烟组血清TNF-α、IL-8、SAA、CRP含量均显著升高（P<0.05）；与模型组比较，艾烟组血清IL-8含量明显降低（P<0.05）；与香烟组比较，艾烟组血清TNF-α、IL-8、CRP含量均显著降低（P<0.05）。结论：艾烟可减少APP/PS1小鼠海马Aβ沉积并减轻炎症反应，提示艾烟对AD脑内神经炎症具有一定的调控作用；香烟则加剧AD脑内病变发展。 |
| English Summary: |
| To observe the effect of moxa smoke and cigarette smoke on hippocampal β-amyloid(Aβ) and serum inflammatory factors in APP/PS1 mice and to investigate the mechanism of moxa smoke against AD from the perspective of neuroinflammation.Methods:Thirty-six 6-month-old male APP/PS1 mice were randomized into model group，moxa smoke group，and cigarette smoke group(n=12/group)，and twelve C57BL/6 mice of the same genetic background were used as blank group.The mice in the moxa smoke and cigarette smoke groups were exposed to the moxa smoke and cigarette smoke，respectively，whose concentration was controlled within 5~15 mg/m3，while the mice in model group and blank group were exposed to normal air.The intervention time of each group was 8 weeks(20 min/day,6 days/week).Based on Congo red staining，Aβ deposition in the hippocampal tissue of mice was observed，and enzyme-linked immunosorbent assay(ELISA) was employed to detect the levels of tumor necrosis factor-α(TNF-α)，interleukin-8(IL-8)，serum amyloid A(SAA)，and C-reactive protein(CRP) in the serum of mice.Results:According to the results of Congo red staining，a large number of Aβ plaques were deposited in the model group and cigarette smoke group，and scattered Aβ particles were occasionally seen in the moxa smoke group compared with the blank group.Serum levels of TNF-α，IL-8，and CRP were higher in the model group(P<0.05) and serum levels of TNF-α，IL-8，SAA，and CRP were higher in the cigarette smoke group(P<0.05) than in the blank group.Serum IL-8 level was lower in the moxa smoke group than in the model group(P<0.05) and serum levels of TNF-α,IL-8，and CRP were lower in the moxa smoke group than in the cigarette smoke group(P<0.05).Conclusion:Moxa smoke can reduce Aβ deposition in the hippocampal region and alleviate the inflammatory response of APP/PS1 mice，suggesting that it has modulating effect on neuroinflammation in the AD brain.In addition，cigarette smoke exacerbates the development of intracranial lesions in AD patients. |
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