To study the protective effect of Bushen Jiedu Decoction against placenta injury and oxidative stress in pregnant mice after radiation.Methods:Fifty female mice and 25 male mice were selected.After successful conception，female mice were divided into a blank control group，a low-dose radiation group，a medium-dose radiation group，a low-dose radiation+Bushen Jiedu Decoction group，and a medium-dose radiation+Bushen Jiedu Decoction group according to the random number table，with 10 mice in each group.Mice in the low-and medium-dose radiation+Bushen Jiedu Decoction groups received Bushen Jiedu Decoction at 13.5 g/(kg·d) for intervention，while those in the blank control group，and low-and medium-dose radiation groups were given the equal volume of normal saline by gavage.After 10 days of intervention by gavage，mice，except for those in the blank control group，underwent γ ray radiation.Specifically，the radiation dose in the low-dose radiation group and the low-dose radiation+Bushen Jiedu Decoction group was 2 Gy，and that in the medium-dose radiation group and the medium-dose radiation+Bushen Jiedu Decoction group was 4 Gy.The pregnancy outcome，general condition of neonatal mice，placental index，and oxidative stress indicators of pregnant mice of the two groups were compared.Results:In the low-and medium-dose radiation groups，the live birth rate was lower and the stillbirth rate was higher than those in the blank control group(P<0.05).In the low-and medium-dose radiation+Bushen Jiedu Decoction groups，the live birth rate was higher and the stillbirth rate was lower than those in the low-and medium-dose radiation groups(P<0.05).Compared with the blank control group，the low-and medium-dose radiation groups showed decreased body mass，body length，and tail length of neonatal mice，and placental index(P<0.05).Compared with the low-and medium-dose radiation groups，the low-and medium-dose radiation+Bushen Jiedu Decoction groups showed increased body mass，body length，and tail length of neonatal mice，and placental index(P<0.05).SOD and GSH-Px levels of pregnant mice in the low-and medium-dose radiation groups were lower than those in the blank control group，while MDA levels were higher(P<0.05).The levels of SOD and GSH-Px in the low-and medium-dose radiation+Bushen Jiedu Decoction groups were higher than those in the blank control group，and the levels of MDA were lower(P<0.05).Conclusion:Bushen Jiedu Decoction has a significant protective effect on the placenta injury of pregnant rats after radiation，which is beneficial to reduce oxidative stress injury and improve the pregnancy outcome of pregnant mice.