| 引用本文:高庆1，李延晖1，谭德慧1，杨云霜2.中药补肾解毒方对辐射后孕鼠胎盘损伤的防护作用及氧化应激的影响[J].世界中医药,2022,(22):. |
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| 中药补肾解毒方对辐射后孕鼠胎盘损伤的防护作用及氧化应激的影响 |
| Protective Effect of Bushen Jiedu Decoction Against Radiation-induced Placenta Injury and Oxidative Stress in Pregnant Mice |
| 投稿时间：2021-01-23 |
| DOI：10.3969/j.issn.1673-7202.2022.22.005 |
| 中文关键词: 辐射防护 补肾解毒方 妊娠结局 氧化应激 |
| English Keywords:Radiation protection Bushen Jiedu Decoction Pregnancy outcome Oxidative stress |
| 基金项目:国家自然科学基金项目（8170151209) |
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| 中文摘要: |
| 目的：研究中药补肾解毒方对辐射后孕鼠胎盘损伤的防护作用及氧化应激的影响。方法：选取50只雌性以及25只雄性小鼠，待雌性大鼠受孕成功后，将其按照随机数字表法分为空白对照组，低剂量辐射组，中剂量辐射组，低剂量辐射+补肾解毒方组，中剂量辐射+补肾解毒方组，每组10只。低剂量辐射+补肾解毒方组和中剂量辐射+补肾解毒方组大鼠予以补肾解毒方灌胃干预，给药剂量为13.5 g/（kg·d）。空白对照组，低剂量辐射组，中剂量辐射组则予以等体积的生理盐水灌胃。各组连续灌胃10 d后，除空白对照组外，其余4组大鼠均予以γ射线照射，其中低剂量辐射与低剂量辐射+补肾解毒方组γ射线照射剂量为2 Gy，中剂量辐射组与中剂量辐射+补肾解毒方组γ射线照射剂量为4 Gy。比较各组孕鼠妊娠结局，仔鼠一般情况以及胎盘指数，孕鼠胎盘氧化应激指标水平。结果：低剂量辐射组与中剂量辐射组孕鼠的活胎率明显低于空白对照组，且死胎率高于空白对照组，而低剂量辐射+补肾解毒方组、中剂量辐射+补肾解毒方组孕鼠的活胎率明显高于低剂量辐射组与中剂量辐射组，且死胎率低于死胎率低剂量辐射组与中剂量辐射组（均P<0.05）。低剂量辐射组与中剂量辐射组仔鼠体质量、身长、尾长以及胎盘指数均明显低于空白对照组，而低剂量辐射+补肾解毒方组，中剂量辐射+补肾解毒方组仔鼠体质量、身长、尾长以及胎盘指数均明显高于低剂量辐射组与中剂量辐射组（均P<0.05）。低剂量辐射组与中剂量辐射组孕鼠超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）水平均低于空白对照组，而丙二醛（MDA）水平高于空白对照组；低剂量辐射+补肾解毒方与中剂量辐射+补肾解毒方组孕鼠SOD、GSH-Px水平均高于空白对照组，而MDA水平低于空白对照组（均P<0.05）。结论：中药补肾解毒方对辐射后孕鼠胎盘损伤的防护作用显著，有利于减轻氧化应激损伤，改善孕鼠妊娠结局。 |
| English Summary: |
| To study the protective effect of Bushen Jiedu Decoction against placenta injury and oxidative stress in pregnant mice after radiation.Methods:Fifty female mice and 25 male mice were selected.After successful conception，female mice were divided into a blank control group，a low-dose radiation group，a medium-dose radiation group，a low-dose radiation+Bushen Jiedu Decoction group，and a medium-dose radiation+Bushen Jiedu Decoction group according to the random number table，with 10 mice in each group.Mice in the low-and medium-dose radiation+Bushen Jiedu Decoction groups received Bushen Jiedu Decoction at 13.5 g/(kg·d) for intervention，while those in the blank control group，and low-and medium-dose radiation groups were given the equal volume of normal saline by gavage.After 10 days of intervention by gavage，mice，except for those in the blank control group，underwent γ ray radiation.Specifically，the radiation dose in the low-dose radiation group and the low-dose radiation+Bushen Jiedu Decoction group was 2 Gy，and that in the medium-dose radiation group and the medium-dose radiation+Bushen Jiedu Decoction group was 4 Gy.The pregnancy outcome，general condition of neonatal mice，placental index，and oxidative stress indicators of pregnant mice of the two groups were compared.Results:In the low-and medium-dose radiation groups，the live birth rate was lower and the stillbirth rate was higher than those in the blank control group(P<0.05).In the low-and medium-dose radiation+Bushen Jiedu Decoction groups，the live birth rate was higher and the stillbirth rate was lower than those in the low-and medium-dose radiation groups(P<0.05).Compared with the blank control group，the low-and medium-dose radiation groups showed decreased body mass，body length，and tail length of neonatal mice，and placental index(P<0.05).Compared with the low-and medium-dose radiation groups，the low-and medium-dose radiation+Bushen Jiedu Decoction groups showed increased body mass，body length，and tail length of neonatal mice，and placental index(P<0.05).SOD and GSH-Px levels of pregnant mice in the low-and medium-dose radiation groups were lower than those in the blank control group，while MDA levels were higher(P<0.05).The levels of SOD and GSH-Px in the low-and medium-dose radiation+Bushen Jiedu Decoction groups were higher than those in the blank control group，and the levels of MDA were lower(P<0.05).Conclusion:Bushen Jiedu Decoction has a significant protective effect on the placenta injury of pregnant rats after radiation，which is beneficial to reduce oxidative stress injury and improve the pregnancy outcome of pregnant mice. |
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