| 引用本文:单萍，张继龙，笱玉兰，罗利俊.柴胡龙骨牡蛎汤下调星形胶质细胞环氧合酶-2/前列腺素E2的表达[J].世界中医药,2022,(23):. |
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| 柴胡龙骨牡蛎汤下调星形胶质细胞环氧合酶-2/前列腺素E2的表达 |
| Chaihu Longgu Muli Decoction Downregulates the Expression of COX-2/PGE2 in Hippocampal Astrocytes |
| 投稿时间：2021-01-11 |
| DOI：10.3969/j.issn.1673-7202.2022.23.011 |
| 中文关键词: 柴胡龙骨牡蛎汤 戊四氮 星形胶质细胞 环氧合酶-2 前列腺素E2 炎症反应 癫痫 |
| English Keywords:Chaihu Longgu Muli Decoction Pentylenetetrazol Astrocytes Cyclooxygenase-2 Prostaglandin E2 Inflammatory reaction Epilepsy |
| 基金项目:国家自然科学基金青年科学基金项目（81703870） |
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| 中文摘要: |
| 目的：探讨柴胡龙骨牡蛎汤对戊四氮（PTZ）诱导的小鼠海马星形胶质细胞环氧合酶-2（COX-2）/前列腺素E2（PGE2）表达的作用。方法:分离培养小鼠海马星形胶质细胞，免疫荧光鉴定胶质细胞原纤维酸性蛋白（GFAP）表达。将细胞随机分为对照组、PTZ组、PTZ+柴胡龙骨牡蛎汤低剂量（PTZ+CLMD 10 mg/L）组、PTZ+柴胡龙骨牡蛎汤高剂量（PTZ+CLMD 20 mg/L）组，酶联免疫吸附试验检测细胞培养液中COX-2和PGE2的浓度；蛋白质印迹法检测细胞内COX-2、PGE2受体（PTGER2）表达水平。结果:免疫荧光显示星形胶质细胞呈现GFAP阳性表达。与对照组比较，PTZ组COX-2和PTGER2蛋白表达水平明显上调，COX-2和PGE2浓度显著升高（P<0.01）；与PTZ组比较，PTZ+CLMD 10 mg/L组和PTZ+CLMD 20 mg/L组COX-2和PTGER2蛋白表达水平显著降低，COX-2和PGE2浓度也显著下降（P<0.01）。且高剂量的柴胡龙骨牡蛎汤效果更明显（P<0.01）。结论:柴胡龙骨牡蛎汤可抑制PTZ诱导的星形胶质细胞内COX-2/PGE2炎症反应通路。 |
| English Summary: |
| To investigate the effect of Chaihu Longgu Muli Decoction(CLMD) on the expression of cyclooxygenase-2(COX-2)/prostaglandin E2(PGE2) in hippocampal astrocytes in mice induced by pentylenetetrazol(PTZ).Methods:Hippocampal astrocytes of mice were separated and cultured.The expression of glial fibrillary acidic protein(GFAP) was identified by immunofluorescence.The cells were randomized into control group，PTZ group，PTZ+low-dose CLMD(10 mg/L) group，and PTZ+high-dose CLMD(20 mg/L) group.The concentration of COX-2 and PGE2 in cell culture was detected by enzyme-linked immunosorbent assay(ELISA)，and the expression of COX-2 and prostaglandin E receptor 2(PTGER2) was measured by Western blotting.Results:Immunofluorescence suggested the expression of GFAP in astrocytes.The levels of COX-2 and PTGER2 were significantly increased and the concentration of COX-2 and PGE2 was significantly raised in PTZ group compared with those in the control group(P<0.01).The levels of COX-2 and PTGER2 were significantly decreased and the concentration of COX-2 and PGE2 was also significantly reduced in PTZ+low-dose CLMD group and PTZ+high-dose CLMD group compared with those in the PTZ group(P<0.01).The effect of high-dose CLMD was more obvious(P<0.01).Conclusion:CLMD can inhibit COX-2/PGE2 inflammatory reaction pathway in PTZ-induced astrocytes. |
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