世界中医药
文章摘要
引用本文:柴爽,杨岩冰,马江涛,郭云鹏,滕军燕,秦娜,张虹.仙灵骨葆胶囊治疗绝经后骨质疏松症的生物信息学和网络药理学分析和实验验证[J].世界中医药,2022,(24):.  
仙灵骨葆胶囊治疗绝经后骨质疏松症的生物信息学和网络药理学分析和实验验证
Bioinformatics and Network Pharmacology Analysis of Xianling Gubao Capsules in the Treatment of Postmenopausal Osteoporosis and Experimental Verification
投稿时间:2020-12-01  
DOI:10.3969/j.issn.1673-7202.2022.24.005
中文关键词:  仙灵骨葆胶囊  绝经后骨质疏松症  生物信息学  网络药理学  成骨细胞  破骨细胞  血管形成  作用机制
English Keywords:Xianling Gubao Capsules  Postmenopausal osteoporosis  Bioinformatics  Network pharmacology  Osteoblasts  Osteoclasts  Blood vessel formation  Mechanism
基金项目:国家自然科学基金面上项目(82174413);河南省自然科学基金青年科学基金项目(202300410555,222300420198)
作者单位
柴爽,杨岩冰,马江涛,郭云鹏,滕军燕,秦娜,张虹 河南省洛阳正骨医院/河南省骨科医院郑州450016 
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中文摘要:
      目的:基于生物信息学和网络药理学挖掘仙灵骨葆胶囊(XLGB)治疗绝经后骨质疏松症(PMOP)的活性成分和作用机制。方法:采用生物信息学挖掘PMOP相关靶点,采用网络药理学筛选XLGB的活性成分并预测作用靶点,阐明XLGB治疗PMOP的相关靶点,富集分析其信号通路以探究分子机制。动物实验采用去卵巢SD大鼠动物模型,XLGB灌胃3月,检测骨密度,血清活性氧水平,TUNEL染色检测骨组织凋亡情况,蛋白质免疫印迹法检测骨组织内凋亡相关蛋白B细胞淋巴瘤2(Bcl-2)、Bcl-2相关X蛋白(Bax)表达量。结果:筛选出差异表达基因883个,筛选出XLGB活性成分119种,共预测1 734个靶蛋白。通过对XLGB和PMOP共有的26个共同靶点分析显示,AKT1、NFKB1、BAX等靶点及细胞凋亡、破骨细胞分化、cAMP、AMPK等信号通路起重要作用。XLGB组骨密度明显改善,血清活性氧水平降低,TUNEL染色显示XLGB可减少骨组织凋亡情况,提高抗凋亡蛋白Bcl-2、降低促凋亡蛋白Bax的水平。结论:XLGB治疗PMOP具有多成分、多靶点的特点,与调控细胞凋亡密切相关。
English Summary:
      To explore the active ingredients of Xianling Gubao Capsules(XLGB) and the underlying mechanism in the treatment of postmenopausal osteoporosis(PMOP) based on bioinformatics and network pharmacology.Methods:The related targets of PMOP were obtained by bioinformatics,and network pharmacology was adopted to screen the active ingredients of XLGB and predict the targets.The relevant targets of XLGB in the treatment of PMOP were clarified,and the signaling pathways were enriched and analyzed to explore the molecular mechanism.In animal experiments,the ovariectomized SD model rats were administered with XLGB by gavage for three months.The bone density and serum reactive oxygen species(ROS) levels were detected,and the bone tissue apoptosis was detected by TUNEL staining.The expression levels of B-cell lymphoma 2(Bcl-2) and Bcl-2-associated X protein(Bax) in bone tissues were determined by Western blot.Results:A total of 883 differentially expressed genes(DEGs) and 119 active ingredients of XLGB were screened out,and 1 734 target proteins were predicted.The analysis of 26 common targets of XLGB and PMOP showed that AKT1,NFKB1,BAX,and other targets played an important role in cell apoptosis,osteoclast differentiation,cAMP,AMPK,and other signaling pathways.In the XLGB group,bone density was significantly improved and the serum ROS level decreased.TUNEL staining showed that XLGB could reduce the apoptosis of bone tissues,increase the level of anti-apoptotic protein Bcl-2,and decrease the level of pro-apoptotic protein Bax.Conclusion:XLGB have multi-component and multi-target characteristics in the treatment of PMOP,which is closely related to the regulation of apoptosis.
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