| 引用本文:苗玉迪1，胡星星1，王九菊2，李艳春3.川楝子醇提物抑制急性淋巴细胞白血病细胞增殖的作用机制研究[J].世界中医药,2022,(24):. |
|
| 川楝子醇提物抑制急性淋巴细胞白血病细胞增殖的作用机制研究 |
| Mechanism of Alcohol Extract of Melia toosendan in Inhibiting the Cell Proliferation in Acute Lymphoblastic Leukemia |
| 投稿时间：2022-05-13 |
| DOI：10.3969/j.issn.1673-7202.2022.24.007 |
| 中文关键词: 川楝子醇提物 急性淋巴细胞白血病 增殖 凋亡 Bcl-2关联X蛋白 P53基因 B细胞淋巴瘤-2 线粒体 |
| English Keywords:Alcohol extract of Melia toosendan Acute lymphocytic leukemia Proliferation Apoptosis Bcl-2-associated X P53 gene B-cell lymphoma-2 Mitochondrion |
| 基金项目:国家级自然科学基金项目（81801647）——干扰素ɑ增强HCA587长肽疫苗的抗肿瘤效应及机制研究；陕西省自然科学基础研究计划（2021JM-548）——LncRNA HOTAIRM1在慢粒中的功能及分子机制 |
|
| 摘要点击次数: 205 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的：探究川楝子醇提物抑制急性淋巴细胞白血病（ALL）细胞增殖的作用机制。方法：选取人急性淋巴白血病细胞T淋巴细胞（CCRF-CEM)细胞株及无特定病原体（SPF)级6周SD品种的雌性大鼠12只，以不同浓度梯度川楝子醇提物作用24 h、48 h、72 h，观察其对CCRF-CEM细胞的抑制及凋亡的影响。结果：16 mg/L、80 mg/L、400 mg/L川楝子醇提物作用ALL细胞的不同时间具有明显的抗增殖作用。相较于对照组，于同一时间点，不同质量川楝子醇提物的吸光度值明显不同，且随着质量的增加吸光度值逐渐降低（P<0.05）。与对照组比较，16 mg/L、80 mg/L、400 mg/L川楝子醇提物作用外周血淋巴细胞同一时间段的给药吸光度值比较差异无统计学意义（P>0.05）。提示不同质量浓度对外周血淋巴细胞无明显的不良反应。于同一时间点，与对照组比较，不同质量浓度川楝子提取物的B细胞淋巴瘤-2（Bcl-2）基因、Bcl-2相关X蛋白（Bax）基因、P53基因表达明显不同（P<0.05）；Bax基因随质量浓度的增加而增加，Bcl-2基因表达随质量浓度的增加而降低，24 h 400 mg/L川楝子醇提物的P53基因表达达到最高，而作用于ALL细胞48 h后，基因表达呈上升趋势（P<0.05）。结论：川楝子醇提物可通过线粒体介导抑制白血病CCRF-CEM细胞增殖，并且呈浓度及时间的依赖性。 |
| English Summary: |
| To explore the mechanism of alcohol extract of Melia toosendan in inhibiting cell proliferation in acute lymphoblastic leukemia(ALL).Methods:An CCRF-CEM cell line and 12 SD female rats of SPF grade aging six weeks were selected.The effect of M.toosendan alcohol extract on the inhibition and apoptosis of CCRF-CEM cells was observed after the treatment with M.toosendan alcohol extracts of different concentrations for 24 h，48 h，and 72 h.Results:M.toosendan alcohol extracts of different concentrations(16，80，and 400 mg/L) for treatment for different time periods showed an obvious effect on the inhibition of the proliferation of CCRF-CEM cells.Compared with the control group at the same time point，the absorbance values of M.toosendan alcohol extracts of different concentrations were different，and the absorbance values gradually decreased with the increase in quality(P<0.05).Compared with the control group，there was no significant difference in the absorbance of 16，80，and 400 mg/L alcohol extracts of M.toosendan on peripheral blood lymphocytes at the same time(P>0.05)，suggesting that alcohol extracts of different mass concentrations had no obvious toxic effect on peripheral blood lymphocytes.Compared with the control group at the same time point，the expression of B-cell lymphoma-2(Bcl-2)，Bcl-2-associated X(Bax)，and P53 genes in the alcohol extract of M.toosendan groups of different concentrations was different(P<0.05).The gene expression of Bax increased with the increase in mass concentrations，while the Bcl-2 expression level decreased with the increase in mass concentration.The P53 gene expression level in the 400 mg/L M.toosendan alcohol extract group peaked at 24 h，while the gene expression showed an upward trend after the treatment of ALL cells for 48 h(P<0.05).Conclusion:The alcohol extract of M.toosendan can inhibit the proliferation of CCRF-CEM cells through mitochondria in a concentration-and time-dependent manner. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
