| 引用本文:黄鹤1，陈腾飞2，李晗2，李林1，高云航2，宋玲2，侯红平2，张广平2，杨红1，叶祖光2.基于液质联用法测定乌头碱类生物碱在大鼠体内药代动力学研究[J].世界中医药,2022,(24):. |
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| 基于液质联用法测定乌头碱类生物碱在大鼠体内药代动力学研究 |
| Pharmacokinetics of Aconitine Alkaloids in Rats Based on Liquid Chromatography Coupled with Mass Spectrometry |
| 投稿时间：2021-03-31 |
| DOI：10.3969/j.issn.1673-7202.2022.24.008 |
| 中文关键词: 液相色谱-串联质谱 乌头碱 次乌头碱 苯甲酰乌头原碱 药代动力学 |
| English Keywords:Liquid chromatography coupled with tandem mass spectrometry(LC-MS/MS) Aconitine Hypaconitine Benzoylaconitine Pharmacokinetics |
| 基金项目:国家重点研发计划项目（2018YFC1708200,2018YFC1708204）；内蒙古自治区科技重大专项（2019ZD004） |
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| 中文摘要: |
| 目的：建立同时测定大鼠血浆样品中乌头碱、次乌头碱、苯甲酰乌头原碱含量的液相色谱-串联质谱(LC-MS/MS)方法，并用于制草乌给药后，3种成分在大鼠体内的药代动力学研究。方法：以小檗碱为内标，采用Shim-pack Velox SP-C18（100 mm×2.1 mm，2.7 μm）色谱柱，流动相为水-甲醇-乙腈（0.05%甲酸），流速0.3 mL/min，质谱采用电喷雾离子源（ESI），正离子模式，选择反应监测（MRM）方式。血浆样品采用有机试剂沉淀蛋白法。结果：乌头碱、次乌头碱、苯甲酰乌头原碱成分的线性关系良好（r≥0.999 0），日内和日间精密度符合生物样品分析要求，相对标准偏差（RSD）均小于15%。结论：此方法灵敏度高、专属性强，适用于测定乌头碱、次乌头碱、苯甲酰乌头原碱的浓度以及药代动力学研究。 |
| English Summary: |
| To establish a liquid chromatography coupled with tandem mass spectrometry(LC-MS/MS) method for simultaneous determination of aconitine，hypaconitine and benzoylaconitine in rat plasma，and to study the pharmacokinetics of the three components in rats after administration of Radix Aconiti Kusnezoffii Praeparata.Methods:Berberine was used as internal standard，and chromatographic separation was performed on a Shim-pack Velox SP-C18(100×2.1 mm，2.7 μm) with water-methanol-acetonitrile(0.05% formic acid) as the mobile phase at the flow rate of 0.3 mL/min.The mass spectrometry was carried out by electrospray ionization(ESI)，positive ion mode and multiple reaction monitoring(MRM).The plasma samples were precipitated by organic reagent.Results:The linear relationship of aconitine，hypaconitine and benzoylaconitine was good(r≥0.999 0).The intra-day and inter-day precision met the requirements of biological sample analysis，with RSD less than 15%.Conclusion:This method is highly sensitive and specific，and it is suitable for the determination of aconitine，hypaconitine and benzoylaconitine and the study of the pharmacokinetics. |
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